Procollagen type

Type I collagen molecules are heterotrimeric molecules with an a chain composition of two al (I) chains and one o 2(I) chain from the COLlAl and COLl A2 genes, respectively. This a chain composition is written as [a 1(1)] 20 2 (I). A type I collagen molecule is synthesized as a procollagen type I molecule. The N- and C-terminal propeptides are cleaved as part of the processing in tissues and are called N- and C-propeptides, respectively. The human pro-o (I) and pro-o 2(I) have 246 and 247 residues in their C-propeptides, respectively. The C-propeptide contains interchain disulfide bonds, which are important for the stabilization of the three a chains in the endoplasmic reticulum (ER). 

Procollagen type I carboxy-terminal propeptide (PICP) Procollagen type I amino-terminal propeptide (PINP) Procollagen type III amino-terminal propeptide (PIIINP) Bone resorption Blood... 

Blood Alkaline phosphatase (bone-specific) Osteocalcin Procollagen type I carboxy-terminal propeptide (PICP) Procollagen type I amino-terminal propeptide (PINP) Procollagen type III amino-terminal propeptide (PIIINP) Blood Acid phosphatase (acid-resistant) Type I collagen carboxy-terminal telopeptide (ICTP) Urine Calcium Hydroxyproline Cross-linked peptides (pyridinium and deoxypyridinoline)... 

J2. Jensen, C. H. H., Hansen, M., Brandt, J., Rasmussen, B., Jensen, P. B., and Teisner, B., Quantification of the N-terminal propeptide of human procollagen type I (PINP) Comparison of ELISA and RIA with respect to different molecular forms. Clin. Chim. Acta 269, 31-41 (1998). 

P7. Pedersen, B. J., and Bonde, M., Purification of human procollagen type I carboxyl-terminal propeptide cleaved as in vivo form procollagen and used to calibrate a radioimmunoassay of the propeptide. Clin. Chem. 40, 811-816 (1994). 

Procollagen type 1 carboxy-terminal Type 1 collagen... 

Procollagen type 1 amino-terminal telopeptide (ICTP)... 

Tsutsurai M, Takase S, Urashima S, Ueshima Y, Kawa-liara H, Takada A. Serum markers for hepatic fibrosis in alcoholic liver disease Which is the best marker, type III procollagen, type IV collagen, laminin, tissue inhibitor of metalloproteinase, or prolyl hydroxylase Alcohol Clin Exp Res 1996 20 1512-7. 

Cho, J-J., and Y-S. Lee. 1998. Enzyme linked immunosorbent assay for serum procollagen type III peptide in rats with hepatic fibrosis. Journal of Veterinary Medical Science 60 1213-1220. 

FIGURE 25.2 Effects of NIPP-1 and NIPP-2 on procollagen synthesis in LX-2 cells. Ten percent FBS was used for the tests to eliminate the interference of serum with the produced protein. Procollagen type IC-peptide assay was done after 24 h (A) and 48 h (B) of treatment. Each value was expressed as the mean SD of triplicate experiments. P< 0.05, P< 0.01, and p< 0.001 as compared with TGF-pi-treated cells. P< 0.001 as compared with non-TGF-pi-treated cells. 

A commercially available thiol-containing agarose has been applied to the purification of procollagen type II from Swarm chondrosarcoma of rzt. ... 

Increased amounts of procollagen type I are deposited in tretinoin treated photoaged dermis, both intracellulary and extracellulary within 10-12 months [51] and may result from transcription up-regulation. Moreover, at an ultra-structural level, collagen fibers are both far more numerous and more orderly in treated skin [49, 50, 64]. 

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