Procedure 4.7.b Determination of AH and AS for the

Load one of the stopped-flow syringes with Stock 1 and the other with the Reaction 1 

Observe the absorbance decrease at 444 nm (the absorbance maximum for the [Fe(CN)5(H20)]3 complex) over at least three reaction half lives at 30 °C. Carry out three trial runs. 

For each mn, plot ln Aoo — A, vs t, where A, is the measured absorbance at time, t. Obtain the pseudo-first-order rate constant, k()B (s 1) from the slope of the plot. 

Report the average of your three trials for each temperature run. 

Obtain the formation rate constant, kt (m 1 s-1), using equation (4.24). 

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