Principles and applications of fluorescence spectrofluorimetry

Note A spectrofluorimeter is quite different from a luminometer (which measures chemiluminescence), and it is important not to confuse the two. Unlike fluorescence, chemiluminescence relies on a chemical reaction between, for example, firefly luciferase and luciferin and molecules in the sample. For measures of chemiluminescence, molecules promoted to an excited state as a result of the reaction return to the ground state with emission of light, which can be measured. 

Understanding Bioanalytical Chemistry Principles and applications Victor A. Gault and Neville H. McClenaghan 2009 John Wiley Sons, Ltd 

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