Pressure cooker retrieval

Taylor CR, Shi S-R, Chen C, et al. Comparative study of antigen retrieval heating methods microwave, microwave and pressure cooker, autoclave, and steamer. Biotech. Histochem. 1996 71 263-270. 

Antigen retrieval place sections in a Coplin jar with antigen retrieval solution of choice (e.g. 10 mM citrate acid, pH 6) and heat at 90°C 110°C (depending on tissue) in a microwave, steamer, domestic pressure cooker or autoclave. See Sect. 6.1.1. 

Put grids in Eppendorf microtubes filled with an antigen retrieval solution (10 mmol/1 citric acid, pH 6.0) and heat them in a domestic pressure cooker at boiling point for 2 5 min (Buchwalow et al. 2004, 2005). The cap of the microtube must have a hole bored by a needle in order to prevent the loss of the material in the process of bubble formation when boiled in a retrieval solution. 

The pressure cooker-microwave heating method is simpler than the autoclave procedure and more efficient than microwave heating alone. The pressure cooker does not require checking the level of the antigen retrieval solution during heating in the microwave oven, and a large number of slides can be loaded simultaneously. In addition, the pressure... 

The most commonly accepted point of view is that heat is responsible for unmasking the epitopes. In fact, Battifora (1996) has introduced the phrase heat-induced epitope retrieval (HIER). Heating at KXPC is a powerful treatment that can unmask hidden, buried, or crosslinked epitopes. Heat can be provided not only by a microwave oven, but also by an autoclave, a pressure cooker, steam, or a hot plate. A consensus on which method of heating is most effective in the retrieval of all types of epitopes is lacking. Therefore, some... 

PRESSURE COOKER-EDTA-ASSISTED ANTIGEN RETRIEVAL... 

Target Retrieval Solution (Dako S1700) is diluted 1 10 with distilled water and adjusted to pH 6.0. ThinPrep smears are rehydrated in 95% ethanol and distilled water for 5 min each. The smears are placed in a plastic slide container with sufficient retrieval solution. The container is immersed in a sufficient quantity of warm water inside the microwave pressure cooker (Dako, DO300X) and heated in a microwave oven for 20 min at full power until steam is generated. The smears, along with retrieval solution, are allowed to cool for 10 min. The smears are removed and rinsed with warm water, followed... 

Miller, R. T., and Estran, C. 1995. Heat-induced epitope retrieval with a pressure cooker. Appl. 

Pressure cookers set to approximately 103 kPa/15 psi will achieve a temperature of approximately 120 °C at full pressure. Alternatively, setting at 125 °C can be used for antigen retrieval. Stainless steel pressure cookers are recommended as the aluminium models are susceptible to corrosion by some retrieval solutions. As an alternative, individual plastic container(s) can be filled with retrieval solution and placed in reagent grade water in the pressure cooker pan. 

Fill the pressure cooker with enough retrieval solution to cover slides. Alternatively, fill individual plastic container(s) with retrieval solution and add at least 500 mL of reagent grade water to pressure cooker chamber. 

Boil for 30 seconds to 5 minutes, allow the pressure cooker to cool for 20 minutes prior to opening. (Note Vent any residual pressure before opening). Open programmable pressure cooker when antigen retrieval procedure is completed. 

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