Preliminary Analysis of PrP

Do processes such as RNA-editing cause changes in the amino acid sequence  

Do any identified modifications possess unusual features that could explain the pathogenic nature of PrP  

The Syrian hamster PrP gene sequence was already known from partial purification of PrP 27-30, Edman iV-terminal sequencing, cloning of a cDNA, and subsequent similar studies on full length PrP and 

GENFTETDIK GQGGGTHNQWNKPSKPK ESQAYYDG GQGGGTHNQWNK ESQAYYDGRRS-GP/ pEHTVTTTTK QHTVTTTTK. 

Experiments with synthetic peptides established that the hydrophilic tetrapeptide Thr-Asn-Met-Lys was not retained on the C18 HPLC column. The HPLC column flow-through from the Lys-C digest was derivatized to add a cholate group to the Atterminus. The increased hydrophobicify allowed the derivatized peptide to be repurified by HPLC and analyzed by 

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