Precision and sensitivity of the HPLC method

Reproducibility of our HPLC method for lipoprotein analysis is excellent. The elution volume Is reproducible within 0.01 ml for each lipoprotein peak in the case of the individual lipoproteins or serum lipid monitoring system. Reproducibility of the concentration of lipid in each lipoprotein class obtained as described in Section 5 is very high. The standard deviation from the mean value for each lipoprotein fraction was 0.1 to 0.4 mg/dl, and the coefficient of variation (Cv) is less than 2 % for the concentration of PL as presented in Table 3. 

The detection limit for each lipid component in serum lipoproteins in our method using the enzymatic reaction is as follows 0.5 pg for TC (20), 1.0 pg for PL (23) and TG (21). On the other hand, the detection limit of protein by Aggg is about 3 pg. The typical example of the elution patterns of cholesterol 

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