Precipitation reactions. Color Plate

With the permission of your instructor, carry out the following experiment. In a beaker, mix equal volumes of 0.001 M NH4SCN and 0.001 M FeCE (the latter solution must be acidified with concentrated HNO3 at a ratio of 4 drops/L to prevent the precipitation of Fe(OH)3). Divide solution in half, and add solid KNO3 to one portion at a ratio of 4 g per 100 mL. Compare the colors of the two solutions (see Color Plate 3), and explain why they are different. The relevant reaction is... 

Procedure. To test for aluminum in the presence of other metals which give color or precipitation reactions with morin, the test solution is treated with excess 2 N potassium hydroxide and filtered. A drop of the filtrate is acidified on a black spot plate with 2 N acetic acid, and a drop of a saturated morin... 

Procedure. The reaction is conducted on a spot plate. A drop of the test solution is treated with a drop of 25 % potassium cyanide solution and a drop of a mixture of 25 % pyridine and 2 % benzidine hydrochloride solution. If elementary chlorine or bromine is present, a red precipitate or color results. Limit of Identification 2.6 y chlorine or bromine... 

Procedure. The test is conducted in a small quartz crucible. One or two mg of the finely ground sample are mixed with several mg of zinc chloride and a drop of water and taken to dryness. The evaporation residue is then heated to faint redness in the covered crucible. The cold residue is stirred with 1 or 2 drops of hot water and the suspension is transferred to a conical tube and centrifuged. A drop of the clear liquid is treated on a spot plate with a freshly prepared 0.2 % solution of sodium rhodizonate and three drops of concentrated ammonia. The mixing is accomplished by blowing on the liquid. A violet precipitate or color appears if calcium is present. A comparison test is required when tiny quantities of calcium are suspected. The same amount of zinc chloride should be used in both cases since it often gives a slight self-reaction due to slight contamination with calcium and lead. 

After terminating the coupling reaction with ascorbic acid, reaction mixture clarified by precipitating protein with ethanol Turbidity decreased by final addition of caffein, sodium benzoate, and Teepol micromethod After the coupling reaction with ascorbic acid is terminated, azo color is extracted and read low blank the extract can be applied directly to a thin-layer plate and be separated rapidly to determine the ratio BMC BDC Micro versions assay artificially activated enzyme... 

This redox reaction (1) proceeds so rapidly that if a drop of 0.01 % bismuth solution is mixed on a spot plate with a drop of alkaline stannite solution, there is an immediate precipitation of metallic bismuth in the form of black flocks. When the same experiment is made with 1 % lead acetate solution, after 3-10 minutes standing there is a slight reduction to lead, which is revealed by a light brown coloration. This proves that the redox reaction ... 

Procedure I. In the depression of a spot plate, mix 15 drops of potassium ferricyanide solution with 10 drops of a solution of diethylaniline a) or b). The sulfuric acid solution remains yellow and clear during the time of observation of the reaction (6 minutes). On addition of a solution containing zinc, the yellow changes to light brown to red-brown, and a precipitate or cloudiness appears. The phosphoric acid solution is dark yellow to red-yellow, and also remains clear during the observation time (5 minutes). Upon adding a drop of a solution containing zinc, the color becomes redder or dark red-brown, and a precipitate or cloudiness is observed. 

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