Practical experimental methods for whole-cell biotransformations using bacteria and fungi

3 Practical experimental methods for whole-cell biotransformations using bacteria and fungi 

From a pragmatic viewpoint, only those biotransformations that result in the majority of the desired product accumulating extracellularly should be considered practical propositions by experimenters other than those experienced in this area of biotechnology. Interestingly, the majority of biotransformations meet this criterion. 

In order to accomplish any small-scale microbial whole-cell biotransformation, many, if not all, of the following key questions need to be resolved  

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