Postsynaptic density protein-95 PSD

For years, it was thought that the cytosolic carboxyl termini as well as various intracellular loops of the 5-HT receptors bind to FRAPs. Efforts from our lab to identify FRAPs utilized yeast two-hybrid screens see Fig. 1 for an overview), phage display see Fig. 2 for an overview), and direct biochemical approaches (8-13). These studies led to the discovery of many 5-HT2A receptorinteracting proteins, including caveolin-1 (Cav-1), arrestin-2 (Arr-2), arrestin-3 (Arr-3), microtubule-associated protein-1A (MAP-1A), and postsynaptic density protein-95 (PSD-95) see Table 1). 

Riefler GM, Balasingam G, Lucas KG, Wang S, Hsu S-C, et al. 2003. Exocyst complex subunit sec8 binds to postsynaptic density protein-95 (PSD-95) A novel interaction regulated by cypin (cytosolic PSD-95 interactor). Biochem J 373 49-55. 

Synapse formation and maturation in different in vitro models can be evaluated by immune-staining of presynaptic markers (e.g., synaptophysin and synapsin) [93] and postsynaptic markers (e.g., postsynaptic density protein-95 (PSD-95) and 41 IB) [94], Moreover, neurotransmitter release can be studied by measuring the uptake and release of fluorescent dyes during presynaptic activity (e.g., FM1-43) [95], In spontaneously electrically active in vitro models, electrophysiological techniques can be another option to evaluate the neuronal network development and functional synapses (see paragraph below). Any disturbance of the synaptogenesis process and function induced by chemical exposure would most likely alter the electrical activity that can be used as a sensitive readout for in vitro DNT/neurotoxicity evaluation [12, 96]. 

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