Polysaccharides, from agar

If branching is (1 —> 3), no glycol grouping will be present in any central unit and it may be seen that reaction will only occur on the terminal units. Such is the case with the polysaccharide laminarin, with the glucan of yeast membrane, and with a galactose-containing polysaccharide isolated from agar.146-148... 

M. Duckworth and W. Yaphe, Preparation of agarose by fractionation from the spectrum of polysaccharides in agar, Anal. Biochem. 44 (1971) 636-641. 

J. R. Turvey and E. L. Williams, The agar-type polysaccharide from the red alga Ceramium rubrum, Carbohydr. Res., 49 (1976) 419 125. 

A. I. Usov and E. G. Ivanova, Polysaccharides of algae. 31. Enzymic hydrolysis of agar-like polysaccharide from the red seaweed Rhodomela larix (Turn.) C. Ag, Sow J. Bioorg. Chem., 1 (1981) 572—579 (English translation from Bioorg. Khim., 7 (1981) 1060-1068). 

E. Marinho-Soriano, Agar polysaccharides from Gracilaria species (Rhodophyta, Gracilariaceae), J. Biotechnol., 89 (2001) 81-84. 

Fig. 2.—Agar-diffusion pattern of immune serum (Ab) and antigenic polysaccharides from Streptococcus faecalis. Wells 1 and 2 contain a tetraheteropolysaccharide, wells 3 and 4 contain a diheteropolysaccharide, and wells 5 and 6 contain a mixture of the two antigenic polysaccharides. [From J. H. Pazur in Carbohydrate Analysis A Practical Approach, (M. F. Chaplin, J. F. Kennedy, eds.) IRL Press, Oxford, England, 1986, pp. 55-96, by permission of Oxford University Press.]...

Fig. 23.—Affinity chromatography of immune serum produced by immunization of a rabbit with nonviable cells of S. faecalis. Inset is the agar diffusion of antibodies and lactosyl polysaccharide from the same organism. (Reprinted with permission from Journal of Protein Chemistry, Volume 6, J. H. Pazur, M. E. Tay, B. A. Pazur, and F. J. Miskiel, pp. 387-399, copyright 1987 Journal of Protein Chemistry.)...

Gels from the natural polysaccharide polymers, agar and agarose, can give good results with nucleic acids. They provide satisfactory... 

Sekkal, M., Huvenne, J.P., Legrand, P., Sombret, B., Mollet, J.C., Mouradigivemaud, A. and Verdus, M.C. (1993) Direct strucmral identification of polysaccharides from red algae by FTIR microspectroscopy. 1. Localization of agar in Gracilaria verrucosa sections. Mikrochim. Acta, 112, 1-10. 

Obt. from agar by partial acid hydrol. and from the polysaccharide of the red seaweed Gloiopeltis furcata. Amorph. solid. [a]i, -15.9 (c, 0.82 in H2O). 

Separation gels are usually made from agar, a polysaccharide extract of seaweed, or other synthetic polymers such as polyacrylamide. The latter find application for high-resolution separations of DNA in the range of tens to hundreds of nucleotides in length. 

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