Polysaccharide hydrazide compounds

Amine functionalities also may be created on polysaccharides (Section 4.3, this chapter). The reducing ends of carbohydrate molecules (or generated aldehydes) may be reacted with small diamine compounds to yield short alkylamine spacers that can be used for subsequent conjugation reactions. Hydrazide groups may be similarly created using f z s-hydrazide compounds (Sections 4.5 and 4.6, this chapter). 

The carbonyl-reactive group on these crosslinkers is a hydrazide that can form hydrazone bonds with aldehyde residues. To utilize this functional group with carbohydrate-containing molecules, the sugars first must be mildly oxidized to contain aldehyde groups by treatment with sodium periodate. Oxidation with this compound will cleave adjacent carbon-carbon bonds which possess hydroxyl groups, as are abundant in polysaccharide molecules (Chapter 1, Sections 2 and 4.4). 

Hydrazide derivatives also may be prepared from a periodate-oxidized dextran polymer or from a carboxyl-containing dextran derivative by reaction with te-hydrazidc compounds (Chapter 4, Section 8). A hydrazide terminal spacer provides reactivity toward aldehyde- or ketone-containing molecules. Thus, the hydrazide-dextran polymer can be used to conjugate specifically glycoproteins or other polysaccharide-containing molecules after they have been oxidized with periodate to form aldehydes (Chapter 1, Section 4.4). 

BODIPY 530/550 C3 hydrazide is 4,4-difluoro-5,7-diphenyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl hydrazide, a derivative of the basic BODIPY structure, which contains two phenyl rings off the No. 5 and 7 carbon atoms and a propionic acid hydrazide group on the No. 3 carbon atom (Molecular Probes). The hydrazide functional group reacts with aldehyde- or ketone-containing molecules to form hydrazone linkages (Fig. 229). The compound may be used to label glycoproteins or other carbohydrate-containing molecules after oxidation of their polysaccharide portions with sodium periodate to yield aldehyde residues. 

The reaction of an arabinogalactan (AG) and its oxidized forms with the antituberculosis preparation isonicotinic acid hydrazide (INAH) was investigated. The chemical composition and certain physicochemical properties of the modified compounds were studied. A correlation was found between the concentration of carboxylic acids in the polysaccharides and the dmg content in the modified compounds. 

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