Polymerase chain reaction single nucleotide polymorphism

SNP on Chip Micro- and Nanofiuidics for Single-Nucleotide Polymorphism Discrimination, Fig. 2 Integrated microfluidic devices for (a) parallel polymerase chain reaction and (b) DNA hybridization (From Erickson and Li [5])... 

Figure 5.10 Schematic representation of analysis of single nucleotide polymorphisms (SNPs) by primer extension and MALDI-TOF-MS. The genomic target region is first amplified by the polymerase chain reaction (PCR). An oligonucleotide primer is then annealed immediately adjacent to the polymorphic site. This primer is extended allele-specifically using a DNA polymerase and a nucleotide mix that leads to a termination of the primer extension reaction either after one or two nucleotide additions. The length of the extension products is determined by the allele present in the analyzed sample. In the depicted case, the...

Abbreviations RFLP, restriction fragment length polymmphism SSP-PCR, polymerase chain reaction using sequence-specific primers SNP, single nucleotide polymorphism. 

Reverse transcriptase polymerase chain reaction Short-acting beta-adrenoceptor agonist Standardized mortality rate Single nucleotide polymorphism Serotonin and noradrenaline reuptake inhibitor Selective serotonin reuptake inhibitor Simian virus 40... 

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