Polymerase chain reaction schematic representation

Figure 3-1. Schematic representation of the polymerase chain reaction. The two strands of the template DNA are represented by the white and black bars. The upper (forward) and lower (reverse) primers are indicated by a hatched and solid white arrow, respectively. The 5 and 3 refer to the corresponding hydroxyl groups on the ribose residue in the DNA backbone, and indicate the directionality of the DNA. DNA polymerases synthesize DNA in the 5 to 3 direction.

Fig. 2. Direct transcriptional template generation from cDNA library by using the split-primers polymerase chain reaction (PCR) technique. (a-c) A schematic representation of the split-primers design for equipping the cDNA sequences with required UTRs, where b and c are expected PCR-generated DNAs and mRNA, respectively, (cl) Split-primer PCR-generated products.

FIGURE 2.2 Schematic representation of a polymerase chain reaction (PCR). The three-step cycle of denaturation, annealing, and extension is repeated 35 to 40 times to generate more than 10 copies of the targeted DNA fragment. 

Figure 5.10 Schematic representation of analysis of single nucleotide polymorphisms (SNPs) by primer extension and MALDI-TOF-MS. The genomic target region is first amplified by the polymerase chain reaction (PCR). An oligonucleotide primer is then annealed immediately adjacent to the polymorphic site. This primer is extended allele-specifically using a DNA polymerase and a nucleotide mix that leads to a termination of the primer extension reaction either after one or two nucleotide additions. The length of the extension products is determined by the allele present in the analyzed sample. In the depicted case, the...

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