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Polymerase chain reaction analysis variation

Analysis of DNA Sequence Variation at Population Level by Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis... [Pg.419]

More recently, DNA amplification by the polymerase chain reaction with thermostable Taq polymerase (Saiki et al. 1988) has replaced cloning to provide substrate for direct analysis of variation. Once a segment of DNA is amplified, variation can be revealed by restriction enzyme digestion (either 4- or 6-cutter) followed by gel electrophoresis and ethidium bromide staining, or by DNA sequencing. [Pg.27]

See other pages where Polymerase chain reaction analysis variation is mentioned: [Pg.265]    [Pg.255]    [Pg.411]    [Pg.114]    [Pg.79]    [Pg.355]    [Pg.295]    [Pg.25]    [Pg.961]    [Pg.126]    [Pg.1327]    [Pg.165]    [Pg.165]    [Pg.206]    [Pg.212]    [Pg.108]    [Pg.1224]    [Pg.260]    [Pg.55]    [Pg.44]    [Pg.212]    [Pg.363]   


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