Polyhydroxybutyrate crystallization

Figure 19 A molecular surface representation of the PHA depolymerase from Penicillium funiculosum cemmQ on the mouth of the crevice. The positions of solvent-exposed hydrophobic residues (purple), as well as polar (green) and catalytic triad (cyan) residues, are indicated. A model of the 3HB trimer bound in the crevice is shown as a yellow stick model. Reproduced with permission from Hisano, T. Kasuya, K. Tezuka, Y. etal. The Crystal Structure of Polyhydroxybutyrate Depolymerase from Penicillium funiculosum Provides Insights into the Recognition and Degradation of Biopolyesters, J. Mol. Biol. 2006, 356, 993, Copyright (2006), with permission from Elsevier.

Gordeyev SA, Nekrasov YPJ (1999) Processing and mechanical properties of oriented poly(beta-hyioxybutyrate) fibers. Mater Sd Lett 18 1691-1692 Hisano T, Kasuya K, Tezuka Y, Ishii N, Kobayashi T, Shiraki M, Oroudjev M, Hansma H, Iwata T, Doi Y, Saito T, Mild K (2006) The crystal structure of polyhydroxybutyrate depolymerase from Penicilliumjuniculosum provides insights into the recognition and degradation of biopolyesters. J Mol Biol 356 993-1004... 

Murase T, Suzuki Y, Doi Y, Iwata T (2002a) Non hydrolytic fragmentation of a poly[(R)-3-hydroxy-butyrate] single crystal revealed by use of a mutant of polyhydroxybutyrate depolymerase. 

Numata K, Sato S, Fujita M, Tsuge T, Iwata T, Doi Y (2007) Adsorption effects of poly(hydroxybutyric add) depolymerase on chain-folding surface of polyester single crystals revealed by mutant enzyme and frictional force microscopy. Polym Degrad Stab 92 176-183 Ohura T, Kasuya K, Doi Y (1999) Cloning and characterization of the polyhydroxybutyrate depolymerase gene of Pseudomonas stutzeri and analysis of the function of substrate-binding domains. Appl Environ Microbiol 65 189-197... 

Polyhydroxyalkanoates, Polyhydroxybutyrate, History, Bacterial synthesis. Chemical synthesis. Genetic engineering. Mechanical properties. Thermal transitions. Crystallization, Plasticizers, Thermal degradation. Processing, Applications... 

Nanocomposites based on polyhydroxybutyrate and hydroxyapatite have been proposed for application in bone repair and regeneration. The hydroxyapatite nanoparticles acted as nncleating agent during PHB crystallization. ... 

Fig. 2.7 (a) Spherulites in a thin film of polypropylene (Elaine Ann Perkins, University of Reading, UK, unpublished work, 1985), (b) spherulites in polyhydroxybutyrate-co-valerate (Reprinted with permission from Macromolecules, 2010, 43 4441-4444 Wang Z, Li Y, Yang J, Gou Q, Wu Y, Wu X, Liu Pand Gu Q (2010) Twisting of Lamellar Crystals in Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) Ring-Banded Spherulites Copyright (2010) American Chemical Society, (c) Schematic of sphaulite twisted orientations. Redrawn from Hsieh et al. (2014)... 

Figure 8.1 Change of spherulitic morphology (decrease of spherulite size) of PHB (polyhydroxybutyrate) with increasing content of needle-shaped hydroxyapatite (HA) nanoparticles, crystallized at 90°C content of HA in wt.% ...

Figure 10 shows the change in specific heat capacity and the exothermic heat flow rate during the quasi-isothermal crystallization of polyhydroxybutyrate (PHB). 

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