Polyadenylation states

Salles, F. J., and Strickland, S. (1995). Rapid and sensitive analysis of mRNA polyadenylation states by PCR. PCR Methods and Applications 4, 317-321. 

This reaction was reported for the benzophenone photosensitized degradation of polypropylene (92),polystyrene (95), poly(vinyl alcohol) (94),polyisoprene (95,96),polyurethane (97) and polyadenylic acid (98) In solid state benzophenone also produce an extensive crosslinking of polyethylene (99-105) It has also been found that benzophenone and its derivatives caused an initial rapid oxidation,increasing with ketone concentration (70) Currently the relative importance of singlet oxygen formation in energy transfer reaction between excited benzophenone and molecular oxygen is discussed (92,104) ... 

The steady-state level of RNA in the cell is controlled both by RNA synthesis rates and by RNA turnover. What answer best explains how an alternate polyadenylation site might be utilized to control RNA stability ... 

When in-vitro translation was performed using polyadenylated RNA from developing pea seeds the plastidic phosphorylase form (in its high molecular weight state) was the predominantly translated phosphorylase isozyme whereas the cytosolic counterpart was recovered as a very weak band of radioactivity (data not shown). Again, the apparent molecular weight of the labeled plastidic phosphorylase was lager by 11 kD than that of the purified enzyme form (Fig. 2C lane a). A complete conversion of the precursor to the size of the mature isozyme was achieved when the translation mixture (prior to precipitation with anti-plasti-dic phosphorylase IgG) was incubated with a stromal fraction (precipitate between 40-70% saturation of ammonium sulfate) of isolated pea chloroplasts (Fig. 2C lane b 50 pi stromal fraction per translation mixture, lane c 100 pi stromal fraction). The identity of the precursor was further confirmed by competition experiments (data not shown). 

Maskin is a cytoplasmic polyadenylation element-binding protein-associated factor. Dormant state of maternal mRNAs in immature oocytes is maintained by an abortive interaction of this protein with the eukaryotic initiation factors 4E and 4G. Phosphorylation of maskin promotes the dissociation of this interaction, thereby allows the dormant mRNAs to be translated actively. Aurora phosphorylation of maskin is reported to be involved in protein synthesis in maturing clam and Xenopus oocytes and in centrosome-dep>endent microtubule assembly at mitosis (Kinoshita et al. 2005 Pascreau et al. 2005). 

Nakai, H., Maxwell, I. H., and Pizer, L. I., 1982, Herpesvirus infection alters the steady state levels of cellular polyadenylated RNA in polyoma virus-transformed BHK cells,/. Virol. 42 1131. 

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