Synthesis polyacrylamide gels

P.M. DiGiacomo and C.M. Schramm, "Mechanism of Polyacrylamide Gel Synthesis Determined by u NMR," SPE 11787, presented at the International Symposium on Oilfield and Geothermal Chemistry, Denver, CO, June 1-3, 1983. 

Interest in the chemistry of water-soluble polymers (polyelectrolytes) has been continually increasing during the past 45 years. The tremendous scope of utility for water-soluble polymers has led to a vigorous search for new materials and the rapid development of polyelectrolytes into a dynamic field of industrial research. Growth in this field has been especially rapid since 1960 and today, many companies are engaged in synthesis and applications research on polyelectrolytes that are primarily used in four main marketing areas water treatment, paper, textiles, and oil recovery [1]. Polyacrylamide gel was also used as soil conditioner [2-4]. 

Fig. 1A,B. Application of radical polymerization to the synthesis of neobiopolymers. A Y.C. Lee s approach to galactose-substituted polyacrylamide gels. B The stages of radical polymerization...

Adenosine triphosphate (ATP) is one of the most important cofactors involved in many of the synthetic reactions going on within the cell. Its recent large scale in vitro enzymatic synthesis from adenosine and acetylphosphate is of particular interest. Three enzymes immobilized in polyacrylamide gel were used adenosine kinase, adenylate kinase and acetate kinase (lip. ... 

To illustrate the diversity of strategies available for solid-phase synthesis, several fairly recent protein syntheses can be cited. SRY, an 80-residue DNA binding protein, was syn-thesized[62] on a Pepsyn support (polyacrylamide gel beads) in a continuous flow machine using Fmoc/tBu protection and TBTU/HOBt activation. HPLC gave a homogeneous product with molecular weight 10051 Da (calculated 10033 Da). It bound to DNA, as expected. 

This protocol is for translating the proteins at preparative scale using 50-pL to 2-mL reaction volumes. The level of protein synthesis during a time course was analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and is shown in Fig. 4. 

Fig. 5. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) analysis of 15N-labeled yeast ubiquitin synthesized by wheat germ cell-free protein synthesis system. Left lane molecular weight markers. Middle lane yeast ubiquitin translation solution. Right lane wheat germ alone (control). Yeast ubiquitin is indicated by...

Figure 1. A. The synthetic route that is used for the synthesis of L-Asn-Sepharose CL 6B affinity adsorbent. B SDS-polyacrylamide gel electrophoresis of caL-ASNase purification. Lane 1 E. coli crude extract after induction with 1 mM IPTG Lane 2 EcaL-ASNase purified according to the two-step procedure using cation-exchange and affinity chromatography.

Werdan, K. (1992b). Application ofhigh resolution two-dimensional polyacrylamide gel electrophoresis of polypeptides from culmred neonatal rat cardiomyocytes regulation of protein synthesis by catecholamines. Electrophoresis 13, 748-754. 

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