Poly-LacNAc structures

Figure 6.8 One-pot synthesis of poly-LacNAc structures with two recombinant GTs and In s tr epimerization of UDP-GIc to UDP-Gal 1U).

H., Kren, V., and Elling, L (2009) Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CG L2-galectin-mediated binding of ECM glycoproteins to hiomaterial surfaces. Glycocotg. f., 26, 141 159. 

Fig. 2. iV-glycans of the hybrid type. A triantennary structure with sialylated Af-acetyllactosamine (LacNAc) building blocks B biantennary structure with sialylated or fucosylated poly-LacNAc building blocks. The N-glycan core structure is written in bold letters... 

Fig. 3. O-glycans of the mucin type. A core 2 structure with poly-LacNAc and blood group epitope A B core 4 structure with sialyl Lex epitope. The core structures are written in bold letters...

One-pot cascade reactions of glycan structures are closely connected to the combination of GT modules and enzyme modules for in situ regeneration of nucleotide sugars (Scheme 6.4). The combination of three enzymes resulted in the synthesis of LacNAc [101], which is the starting point for the synthesis of poly-LacNAc and antigenic glycan structures. 

Structurally, the O-polysaccharide chains of H. pylori clinical isolates have a poly-A-acetyl-lactosamine (-LacNAc) chain decorated with multiple lateral a-L-fucose residues forming internal Lex determinants with terminal Lex or Ley units (Fig. 10.4) or, in some strains with additional, D-glucose or D-galactose residues (Moran 2001 a,b, 2008 Monteiro, 2001). Moreover, Lea, Leb, sialyl-Lex, and H-1 antigens have been structurally described in other strains, as well as the related blood groups A and B (Fig. 10.4), but occur in association with Lex and LacNAc chains (Monteiro et al., 2000a,b Heneghan et al., 2000). 

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