Poly incubation temperature

Haba E, Vidal-Mas J, Bassas M, Espuny MJ, Uorens J, Manresa A (2007) Poly 3-(hydroxyal-kanoates) produced from oily substrates by Pseudomonas aeruginosa 47T2 (NCBIM 40044) Effect of nutrients and incubation temperature on polymer composition. Biochem Eng J 35 99-106... 

Fig. 2. Time course of accumulation of HSP mRNA. One jUg of poly(A) RNA isolated from soybean hypocotyls after different times of incubation at 42.5 °C (hs) or at additional times after transfer back to 28 °C after 4 h at the elevated temperature (recovery), were electrophoresed in formaldehyde agarose gels. Blots of these gels were hybridised with a mixture of four cDNAs encoding small soybean HSPs ranging from 15 to 23 kDa. From Schoffl Key (1982).

The ODN adsorption onto cationic microgel poly(N-isopropylacrylamide) particles was reported to be dramatically affected by the salinity of the incubation medium [9] as illustrated in Fig. 6. The observed result was related to (i) the reduction in attractive electrostatic interactions between ODN molecules and the adsorbent and (ii) the drastic effect of ionic strength on the physico-chemical properties of such particles [17, 27]. In fact, the hydrodynamic size, the swelling ability, the electrokinetic properties, and the colloidal stability are dramatically affected by pH, salt concentration, and the medium temperature [27]. 

After storing the samples at each temperature for 1 week, they were rapidly thawed in a warm bath (at 310 K). They were then cultured on poly-L-lysine-coated 24-well plates or the MED probe, which is a plate used to measure nerve impulses, in an incubator (humidified atmosphere including 5% CO2 at 310 K) after replacing the cryoprotectant with the culture medium. The recovering neurons were first examined by phase-contrast microscopy and compared with control samples. The presence of neurons... 

Several years ago, Chakrabarti et al. " described for the first time such a polymerization of RNA inside DMPC vesicles by polynucleotide phosphorylase. In these experiments, ADP was added externally to the enzyme-containing vesicles (this enzymatic reaction does not require template nucleic acids for initiation) and incubated at 23°C, the main phase transition temperature of DMPC. Because of these gel-to-liquid crystalline transitions of the bilayer, the vesicles could take up ADP from the external milieu, and the enzyme could produce poly (A). On the other hand, the enzyme could not leak out because of its size. Similar experiments were also carried out in our group by Walde et al. Here, the vesicles consisted of a single-chain amphiphile (oleic acid/oleate) that forms vesicles at a pH of about 8.0. ADP was shown to permeate across the oleic acid/oleate membrane and was incorporated inside the vesicles by polynucleotide phosphorylase to poly(A). 

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