Poly gene delivery systems

Cherng, J.Y., Talsma, H., Crommelin, D.J.A., Hennink, W.E., (1999). Long term stability of poly((2-dimethylAm.ino)ethyl methacrylate)-based gene delivery systems. Pharm. Res., 16, 1417-1423. 

POLY(L-LYSINE)-BASED GENE DELIVERY SYSTEMS... 

Petersen, H., Fechner, P.M., Martin, A.L., Kunath, K., Stolnik, S., Roberts, C.J., Fischer, D., Davies, M.C., and Kissel, T. (2002) Polyethylenimine-graft-poly(ethylene glycol) copolymers influence of copolymer block structure on DNA complexation and biological activities as gene delivery system. Bioconjugate Chemistry 13 845-854. 

Of the many nonlipidic polycation gene delivery systems developed in the past decades, poly(L-lysine) (PLL) was the hrst polycation used for nonviral gene delivery [34]. Among a vast number of other positively charged polymers, polyethyleni-mine (PEI) has been widely used for nonviral transfection in vitro and in vivo and has an advantage over other polycations in that it combines strong DNA condensation capacity with an intrinsic endosomolytic activity [35-38]. 

E. Verbaan, Colloidal Gene Delivery Systems - In vivo Fate of poly(2-(dimethyl amino) ethyl methacrylate)-based Transfection Complexes, PhD. Thesis, Utrecht University, Holland, 2002, pp. 14-15. 

Some recent reports suggest that Pluronic block copolymers can be used as the components of novel self-assembling gene delivery systems. Astafieva et al. (77) have demonstrated that Pluronic block copolymers can enhance polycation-mediated gene transfer in vitro. A synthetic polycation, poly(A(-ethyl-4-vmylpyridinixun bromide) (PEVP), jmd plasmid DNA, were mixed with 1% P85 to treat the cells. Both the DNA uptake... 

Cationic lipids and cationic polymers are designed as gene delivery systems on the nanoscale. Especially chitosan is under focus as a biodegradable, natural biopolymer, used both as the polyplex and also as a coating material for other polyplexes. Chitosan-coated poly(isohexyl cyanoacrylate) nanoparticles have also been developed for intravenous delivery of siRNA and no evidence of toxicity was observed after intravenous administration for 30... 

Li Z, Zhu S, Gan K et al (2005) Poly-L-lysine-modified silica nanoparticles a potential oral gene delivery system. J Nanosci Nanotechnol 5 1199-1203... 

Lim Y-B, Kim C-H, Kim K, Kim SW, Park J-S (2000) Development of a safe gene delivery system using biodegradable polymer, poly[a-(4-aminobutyl)-L-glycolic acid]. J Am Chem Soc 122 6524-6525... 

In another example, biodegradable cationic polymers based on poly(j3-amino esters) (PbAE) were used for the development of site-specific drug and gene delivery systems. Under acidic conditions PbAE underwent rapid dissolution, releasing its content. For example, it has been observed that... 

Guo C, Chen W, Lin S, Li H, Cheng D et al (2012) Synthesis and characterizatirai of polycation block copolymer poly(L-lysine)-b-poly[N-(NOJ fO-diisopropyl-aminoethyl) aspartamide] as potential pH responsive gene delivery system. Polymer 53 342—349... 

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