Platelets labeled

CD36 or GP IV is a marker that is expressed on both resting and activated platelets. Labeled antibodies can be used that exhibit increased binding to activated platelets. 

H]Inositolphosphate Labeling. Platelets labeled with myo-[3H]inositol phosphates are incubated in Tyrode-gelatin buffer (pH 7.2) containing 1 mM Ca2+ and 10 mM Li+ (all final concentrations) for 10 min. This concentration of Li+ has been shown to inhibit the conversion of inositolphosphates to inositol. As described earlier, the cell preparation is stimulated with I X 10-9 M PAF and at the desired time intervals the reaction is stopped by the addition of 5% trichloracetic acid (w/w) and the mixture is centrifuged at 900 g for 5... 

Ga ° which have been verified as a hydrophilic blood labeling agent (Tc), as tracer for platelets labeling (In) and as radiopharmaceutical agents (In and Ga). 

Various factors influence platelet labeling (Rodrigues and Sinzinger 1994 Table 8.3). Platelet function depends on glycogen as an energy source, glucose being a key sub-... 

Table 8.3. Variables influencing platelet labeling with " Tc-pertechnetate...

Tc-oxine has proved to be a good platelet label. The mean LE (91%) and REC (71%) data of Tc-oxine were found to be comparable to Tc-oxine-sulfate and In-oxine (Angelberger et al. 1981). An LE of >90% was found after an incubation time of only 1 min at 37 °C (Fig. 8.2). Tc-oxine and Tc-oxine-sulfate, however, are not commercially available. 

Becker W, Borner W, Borst U (1988 a) " Tc-hexamethylpropyleneamine-oxime (HMPAO) as a platelet label evaluation of labelling parameters and first in vivo results. Nucl Med Commtm 9 831-842... 

Mortelmans L, Verbruggen A, De Roo M, Vermylen J (1986) Evaluation of three methods of platelet labeling. Nucl Med Comm 7 519-529... 

Rodrigues M, Sinzinger H (1994) Platelet labeling - methodology and clinical applications. Thromb Res 76 399-432... 

Table II. Accumulation of Washed Human Platelets Labeled with Indium on Glass Capillary Surfaces Precoated with Purified Human Albumin, Human Fibrinogen or Bovine Collagen...

Table III. Accumulation of Washed Human Platelets Labeled with 111...

Ginsburg, A.D., Aster, R.H., 1973-1974. Discrepant platelet kinetic data obtained in rats using the platelet labels Cr and [ H]DFP. Haemostasis 2 (6),... 

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