Plate count agar

Microbial contamination, especially by salmonellas, is a risk when sprouts are produced commercially for human consumption. For recombinant protein production, seeds can be washed with water and surface-sterilized using hypochlorite solution. Sprouts can also be surface-sterilized during sprouting, by the addition of mild hypochlorite solution directly into the growth medium. Eventually, the hypochlorite is diluted out with pure water or growth medium. In our experiment on plate count agar [28], the sprouts showed no bacterial growth after sterilization with 1% sodium hypochlorite. 

Variations for 5-hydroxy fluvastatin. Medium for growth on agar plates Plate Count Agar (Fluka/ Sigma Aldrich, Buchs, Switzerland) medium for preculture and main culture glucose 20 g L , soytone (Becton Dickinson) 15 g L , yeast extract 10 g L , pH adjusted to 6.5 with NaOH. Incubation time of main culture before fluvastatin addition 3 days biotransformation period 24 h. 

For the enumeration procedure, each laboratory received 60 capsules and a maximum number of 50 enumerations was requested. The extra capsules were supplied to replace the capsules that were not acceptably dissolved. The capsules were dissolved in 2 rounds each of 30 capsules. For each series also a control was tested, consisting of a Petri-dish containing peptone saline to which no capsule was added. The procedure can be summarised as follows (1) dissolution of a capsule in 5 mL peptone saline solution in a Petri-dish for (45 5) min in an incubator at (38.0 0.5)°C while shaking at ca. 100 rpm (2) repair of Salmonella in Plate Count Agar (incubation time (4 1) h at (37 ... 

These bacteria were cultivated in each product for 24 h at 37 °C and each cultured broth sample was serially diluted into the corresponding aseptic products. The total colony count was determined with Plate Count Agar (MERCK). Bioluminescent Assay... 

Fermenter studies were carried out in a New Brunswick continuous culture apparatus with 15-liter stirred fermenter jars for culture vessel and nutrient reservoirs and a 20-liter carboy for a harvest vessel. Temperature of the culture was maintained at 28°-29°C. Impeller speed was kept at 120 r.p.m. with a 3-inch diameter set of four impeller blades low in the culture, and a second impeller just above the liquid level to aid in foam control. A few drops of Dow Coming Antifoam AF at 25 mg./ml. (emulsion) were added manually as required to control foam. The rate of aeration was 1 to 3 liters per minute. Contamination through the impeller shaft port was prevented by a loose fitting sleeve through which live steam was continually passed (10). Samples taken from the fermenter were streaked on potato dextrose agar, and Bacto plate count agar, incubated for 7-10 days and inspected for contamination. 

Figure 1 Colonies of microorganisms on agar plates after incubation (A) close-up of bacteria pour plate, plate count agar (B) close-up of mold surface plate, oxytetracycline glucose yeast extract agar (C) Salmonella species streak plate, xylose lysine decarboxylase agar.

Total plate counts were run on dehydrated plate count agar (Difco, Detroit, MI). Ten grams of surimi gels were homogenized with 90 mL distilled water using an Osterizer pul-sematic 10 blender (Oster Corporation, Milwakuee, WI) at frappe speed for 1 min. Dilu-... 

Plate Count Agar (PCA) is an agar-based medium used for the enumeration of aerobic bacteria in finished paper. The agar must be melted and cooled (48°C/118°F) prior to use. 

PCA (plate count agar) 20°C-28°C for 5-7 High-nutrient medium [2]... 

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