Plasmids Pluronic

Some recent reports suggest that Pluronic block copolymers can be used as the components of novel self-assembling gene delivery systems. Astafieva et al. (77) have demonstrated that Pluronic block copolymers can enhance polycation-mediated gene transfer in vitro. A synthetic polycation, poly(A(-ethyl-4-vmylpyridinixun bromide) (PEVP), jmd plasmid DNA, were mixed with 1% P85 to treat the cells. Both the DNA uptake... 

Block-graft copolymers synthesized by covalent conjugation of Pluronic and branched PEI were used as materials for preparation of polyplexes. Such polyplexes usually contain three components (i) DNA (ii) Pluronic-PEI conjugate and (iii) free Pluronic (Fig. 7). The formulations were prepared with both plasmid DNA and oligonucleotides (ODN), resulting in stable polyplex dispersion with the particle size in the ranges of 100—200 nm. These polyplexes were used successfully for delivery of plasmid DNA and antisense ODN in vitro... 

Chun et photocrosslinked Pluronic FI27 for sustained delivery of plasmid DNA. The results indicated that release of DNA was dependent on the UV irradiation time and vinyl group-modified hyaluronic acid (HA) amounts, and the released DNA from the HA/Pluronic hydrogels exhibited considerable transfection efficiency without degradation of DNA by the UV irradiation. 

Chun KW, Lee JB, Kim SH, Park TG, Controlled release of plasmid DNA from photo-cross-linked pluronic hydrogels. Biomaterials, 2005, 26, 3319-26. 

Fig. 7 Promoter selective effect of Pluronic P85 on luciferase reported gene expression in skeletal muscle. Groups of five Balb/c mice were injected i.m. with 0.3% P85-formulated (black bars) or nonformulated (white bars) luciferase encoding plasmids driven by CMV, SV40, CRE, AP-1, NF-/cB or p53. Luciferase activity was determined 24h after injections. Data are the percentage of increase of P85-formulated DNA over the naked DNA, mean SEM (n = 10). p < 0.05, n.s. not significant (p = 0.06)...

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