Plasma membrane calcium ATPase

Abstract The plasma membrane calcium ATPase (PMCA) uses energy to pump calcium (Ca2+)... 

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In our experimental model, the palytoxin-induced cellular acidification was completely prevented by inhibitors of the plasma-membrane calcium ATPase, including orthovanadate, lanthanum and an extracellular pH of 8.5. Om results therefore indicate that the plasma-membrane calcium ATPase is involved in the palytoxin-induced intracellular acidification in primary cultures of cerebellar granule cells. We suggest that the increase in intracellular calcium evoked by palytoxin will activate the calcium extrasion mechanisms through the calcium pump, which, in turn, will decrease intracellular pH through countertransport of H ions (Vale-Gonzalez et al. 2007). 

The acidification produced by palytoxin was due to an influx of extracellular calcium, since it was completely abolished in a calcium-free medium. In addition, toxin-induced intracellular acidification was completely prevented by several inhibitors of the plasma membrane calcium ATPase,... 

FIGURE 31.5 Effect of different blockers of the plasma membrane calcium ATPase on the intracellular acidification caused by palytoxin in primary neuronal cultures, as assessed with BCECF-AM. (Data extracted from Vale-Gonzalez, C. et ah, J. Neurosci. Res., 85, 90, 2007.)... 

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