Plasma malondialdehyde determination

Largilliere. C., and Mclancon, S. B. (1988). Free malondialdehyde determination in human plasma by high performance liquid chromatography. Anal. Biochein. 170, 123-126. 

R7. Richard, M. J., Portal, B., Meo, J., Coudray, C., Hadjian, A., and Favier, A., Malondialdehyde kit evaluated for determining plasma and lipoprotein fractions that react with thiobarbituric acid. Clin. Chem. 38, 704-709 (1992). 

Other methods are also used for the determination of plasma/serum levels in vitro lipid peroxidation in erythrocytes (malondialdehyde test) determination of vitamin E concentration in platelets determination of tocopherols in fat tissue (needle biopsy). 

Usually, in reversed-phase systems, ascorbic acid is eluted within 3 to 10 min. However, as extracting biological samples there may be compounds that elute far after ascorbic acid. Gradient elution may be needed to shorten the column purification time between injections. Tanishima and Kita (74) used a stepwise increasing methanol concentration to remove uric acid from the column after plasma samples. Lazzarino et al. (88) separated ascorbic acid, uric acid, xanthine, hypoxanthine, malondialdehyde, and several nucleotide derivatives with ion pairing and methanol gradient in a reversed phase system. The method was used for determining those compounds in ischemic-reperfused rat heart. 

Lord HL, Rosenfeld J, Volovich V, Kumbhare D, Parkinson B. Determination of malondialdehyde in human plasma by fully automated solid-phase analytical deriv-atization. J Chromatogr B 2008 877 1292—8. 

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