Plasma desorption insulin

Californium plasma desorption ( Cf-PD) dates back to 1973 [4-6,22,154-156] and was the first method to yield quasimolecular ions of bovine insulin. [157] Practically, Cf-PD served for protein characterization, a field of application which is now almost fully transferred to MALDI or ESI (Chaps. 10,11). [158]... 

New methods use combined HPLC/Mass spectrometry to identify modified amino acids. Purified recombinant human insulin-like growth factor separated into two peaks on reverse phase HPLC (Cl 8 column/acidified water) even though other methods indicated it was completely pure. Plasma desorption mass spectrometry of the individual peptides detected a single methionine sulfoxide molecule that was sufficient to decrease the hydrophobicity of the whole protein significantly. Most of the oxidation occurred when the secreted fusion protein was cleaved with hydroxylamine under not strictly anaerobic conditions, but about 5% occurred during die . coli fermentation. 

Jespersen, S., Talbo, G., and Roepstorff, P. (1993) Optimization of sample recovery from the nitrocellulose support used in plasma desorption mass spectrometry and its use for multiple analysis of insulin. Biol. Mass Spectrom. 22, 77-83. 

Haakansson, P., I. Kamensky, B. Sundqvist, J. Fohlman, P. Peterson, C. J. McNeal, and R. D. Macfarlane Iodine-127-plasma Desorption Mass Spectrometry of Insulin. J. Amer. Chem. Soc. 104, 2948 (1982). 

Matrix-assisted laser desorption ionization time of flight, see MALDI-TOF Matrix-assisted laser desorption ionization time of flight mass spectrometry, see MALDI-TOF-MS Maximum plasma concentration 51 Maytansanoids, cytotoxic 1172 MCFD2 427 MDRl 58 Meal insulin 1454 Measles-mumps-rubella vaccine 1421 Mecasermin 465... 

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