Plaque lift hybridization

DNA adsorption properties were first studied using a variety of solid supports for classical analysis methods including Southern and Northern transfers, dot-blotting, colony hybridization and plaque-lifts [31,32]. Studies of the interactions between nucleic acids and nitrocellulose revealed that molecular weight, finite macromolecular conformation, ionic forces and weaker forces of attraction all play a role. DNA is retained on nitrocellulose only in... 

When a bacteriophage is used as the cloning vector, the gene library is screened as an array of plaques in a bacterial lawn. A hybridization screening method is used similar to that described for plasmid screening above in this case the replica filter is called a plaque lift. ... 

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