Planorbis comeus

Chen, G. et al.. Observation and quantitation of exocytosis from the cell body of a fully developed neuron in Planorbis comeus, J. Neurosci., 15, llAl, 1995. 

Chien, J. B., Wallingford, R. A., andEwing, A. G, Estimation of free dopamine in the cytoplasm of the giant dopamine cell of Planorbis comeus by voltammetry and capillary electrophoresis, J. Neurochem., 54, 633-638, 1990. 

Early studies by Meulemans et al. used differential pulse voltammetry (see Chapter 11) to measure the concentration of electroactive drugs in the cytoplasm of cholinergic neurons of Aplysia californica and to determine rates of cellular uptake and clearance (108). Another report by Meulemans et al. described a needle tipped glass insulated platinum electrode for detection of intracellular serotonin in metacerebral neurons from Aplysia (109). Again differential pulse voltammetry was used to measure intracellular concentration of serotonin after cell stimulation, after injection of serotonin into the cell, and after extracellular treatment with tryptophan, a serotonin precursor. Serotonin concentration in resting untreated cells was determined to be 0.94 0.61 mM. Chen et al. have also used platinum electrodes with multiple pulse voltammetry to measure dopamine levels in the giant dopamine neuron of Planorbis comeus (110). 

Figure 22. Typical power-time curve for the action of cadmium intoxication on a freshwater snail Planorbis comeus). At the arrow, contaminated water is introduced to the sorption chamber at a higher flow rate to guarantee for a quick and effective water exchange. The bar near the ordinate indicates the standard deviation for a superposition of 3 individual traces [182].

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