Pig aortas

Pig aortas were stripped of adventitia, snap frozen in liquid nitrogen, crushed into a fine powder, resuspended in 0.05 M Tris buffer (pH 7.5) (1 4, w v) and... 

Rat vagus nerve, a, 3-methylene ATP as agonist [54], Guinea-pig aorta, 2-methylthio ATP as agonist [55]. Rat vas deferens [54]. iApparent pKg, derived fi-om a double-reciprocal regression. PPADS interacted with two affinity states of the P2X"Puri" PfO for [ H]a,p -methylene ATP. 

Relaxant responses to 2-methylthio ATP in the carbachol-contracted rat duodenum were antagonized by PPADS (10 - 100 pM) in a concentration-related manner. The resulting Schild plot was linear and its slope (1.02) was not significantly difiTerent fi-om unity, indicating competitive antagonism. The affinity estimate for PPADS in rat duodenum (pA2 = 5.09 Table 3) was very similar to that obtained for pyridoxal-5-phosphate in guinea-pig aorta (5.39), but clearly lower compared to the apparent pKg values obtained for the P2X subtype in rabbit vas deferens (6.34) and rat mesenteric artery (6.38 Table 3). In rat mesenteric arterial bed, the tone of which was raised by methoxamine, vasodilator responses to 2-methylthio ATP were slightly inhibited by 10 pM PPADS (pA2 = 5.46), a concentration which virtually abolished P2X punnoceptor-mediated vasoconstriction. It is noteworthy that PPADS (30 pM) was found to be ineffective at vascular P2Y-punnoceptors in rabbit mesenteric artery and aorta. These results clearly demonstrate that PPADS is less effective at duodenal and vascular P2Y purinoceptors than at P2X-receptors (Table 3). 

However, phospholamban protein was undetectable in pig aorta (Raeymaekers and Jones, 1986 Eggermont etfl/., 1990b). To our knowledge, published data on myometrium are lacking. The amino acid sequence of phospholamban of pig stomach smooth muscle determined from cDNA sequencing is identi-... 

Amphomycin specifically inhibits the formation of dolichyl D-mannosyl phosphate in pig aorta microsomal preparations, but does not inhibit the further transfer of b-mannose from dolichyl D-mannosyl phosphate or from lipid-linked oligosaccharides. However, some of the D-mannosyl residues in the lipid-linked oligosaccharides come directly from GDP-D-mannose without the involvement of dolichyl D-mannosyl phosphate. 

Chambers, J., Forsee, W.T. Elbein, A.D. (1977) Enzymatic Transfer of Mannose from Mannosyl-phosphoryl-polyprenol to Lipid Linked Oligosaccharides by Pig Aorta , Journal of Biological Chemistry, 252, 2498-2506... 

Fig. 10.8 a A 6 mm BC graft as an infiarenal aortic bypass, b Routine histology showing a nice single layer of cells on the interior of the BC surface after 1 month implantation in the pig aorta [78]... 

A structural glycoprotein that participates in fibrogenesis and differentiation of the cells has been isolated from the media of pig aorta. The carboxamido-methylated protein interacted with the soluble, undegraded component of calf-skin collagen. Renin from the kidneys of pigs contains 3.2 residues of 2-amino-2-deoxy-D-glucose for each molecule of protein. ... 

In 1976, Moncada et al. reported that microsomes isolated from rabbit and pig aortas were able to convert endoperoxides into a factor which has strong vasorelaxant and anti-aggregant properties [40]. The product was later identified as the prostacyclin PGI2, which is the major prostanoid produced by... 

The UDP-o-glucuronate glycosaminoglycan jS-D-glucuronyl-transferase activity from the media and intima of pig aorta has been determined by the incorporation of D-[ C]gIucuronic acid into hyaluronic acid and heparan sulphate. ... 

Gordon, E. L., Pearson, J. D., and Slakey, L. L. (1986). The hydrolysis of extracellular adenine nucleotides by cultured endothelial cells from pig aorta. Feed-forward inhibition of adenosine... 

Ikushima, S., I. Muramatsu, and M. Fujiwara Nicotine-Induced Response in Guinea-Pig Aorta Enhanced by Goniopora Toxin. J. Pharmacol, exp. Ther. 223, 790 (1982). 

Gordon, J. L., Martin, W., 1983 Stimulation of endothelial prostacyclin production plays no role in endothelium-dependent relaxation of the pig aorta. Br. J. Pharmac. 80, 179-186. 

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