Physically modified amine columns

A comprehensive survey of 63 low molecular weight saccharides compares the potential of a radially compressed physically modified amine column with that of a cation-exchange column and contains a listing of all the capacity factors (Baust et al., 1983). 

The separation of food and plant saccharides on a silica column using a polyfunctional amine dissolved in the eluent has been demon- 

The new resin-based specialised carbohydrate columns utilise an 8% cross-linked styrene-divinylbenzene copolymer which is functiona- 

Used to produce a strong acid cation-exchanger (Brando et al, 1980 Richmond et al., 1981). These columns are generally operated at an elevated temperature of up to 85 C but lack the ability to successfully resolve components with the same degree of polymerisation, e.g. maltose and sucrose. Using these columns the preferred counter-ion is calcium and they can be initially conditioned with 10 M calcium-acetate followed by the required mobile phase containing 10 M calcium acetate (Baust et al., 1983). Approximately 12 h are required 

Separation of difficult pairs of saccharides such as glucose and galactose, and mannitol and glucitol is strongly dependent upon the acetonitrile concentration (Fig. 11.4.7). The system shown demonstrates good separations for most monosaccharides and for mixed di- and trisaccharides. Nevertheless, certain combinations of saccharides still pose problems which need to be resolved by alternative mobile phases or stationary phases. Other references to the separation of standard mixtures of saccharides can be found in the literature (Vidal-Valverde et al., 1982). 

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