Photosensitized chemiluminescence measurement

The measuring principle of photosensitized chemiluminescence is applied in the Photochem. The principal functional units of the instrument are shown in Figure 3. 

CL was determined in Fluoroskan Ascent FL (j he,rm.oLabsystems, Finland) at 25 °C and 37 °C in phosphate buffer pH 7.6. Chemiluminescent reaction was initiated by the injection of hydrogen peroxide in solution of CL compound in the presence of CUSO4 or HRP. The solution of NaOCl is quite unstable at room temperature. In order to minimize the effect of NaOCl decomposition, the stock solution was defrosted immediately before priming the automatic dispenser and solution was kept on ice. The photodynamic effect was initiated by the irradiation of the solution of photosensitizer by a semiconductor laser (Lasotronic pocket therapy laser, power 50 mW, wavelength 670 nm). All manipulations with chlorophyll a and /0-carotene were done in the dark room and the extracts were kept at -20 °C to minimize degradation processes. All measurements were performed in a volume of 2(X) pL. 

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