Photonic triggering

Fluorescent measurement uses the luminescence of fluorescence that is mostly found as an optical phenomenon in cold bodies, in which the molecular absorption of a photon triggers the emission of another photon with a longer wavelength. The energy difference between the absorbed and emitted photons ends up as molecular vibrations or... 

The smaller amount of neurons driven by the more numerous cones points to a complex wiring of these receptors located in the second layer of the retina. The inner nuclear layer connects the photoreceptors with the ganglion cells and consists of bipolar, amacrinal, and horizontal cells. Bipolar cells can be divided into ON and OFF bipolars. The OFF bipolar cells inhibit the release of neurotransmitters after a photon triggered increase in electrical potential whereas the ON bipolar cells stimulate the release of neurotransmitters. This allows the visual system to build differential signals already in the first processing stage (see Fig. 5). 

Solar energy conversion processes start from photon-matter interactions and a photon triggered chemical reaction almost always generates changes in nuclear geometry of those involved. The availability of high repetition rate, intense and... 

Brunei C, Lounis B, Tamarat P and Orrit M 1999 Triggered source of single photons based on controlled single molecule fluorescence Phys. Rev. Lett. 83 2722-5... 

The reaction mechanisms of plasma polymerization processes are not understood in detail. Poll et al [34] (figure C2.13.6) proposed a possible generic reaction sequence. Plasma-initiated polymerization can lead to the polymerization of a suitable monomer directly at the surface. The reaction is probably triggered by collisions of energetic ions or electrons, energetic photons or interactions of metastables or free radicals produced in the plasma with the surface. Activation processes in the plasma and the film fonnation at the surface may also result in the fonnation of non-reactive products. 

Fig. 6.3.11 Chromatography of an extract of the eye light organs of Symplecto-teuthis luminosa on a column of Superdex 200 Prep (1x27.5 cm) in 20 mM phosphate buffer, pH 6.0, containing 0.6 M NaCl, at 0°C (monitored at 280 nm). Each fraction (0.5 ml) is measured for the initial intensity of H202/catalase-triggered luminescence and the content of dehydrocoelenterazine measured as coelenterazine after NaBH4-reduction 1LU = 6 x 108 photons.

UV-Vis spectroscopy may also provide valuable information if small molecules are studied. However, the photochemical sensitivity of many sulfur-containing molecules may trigger changes in the composition of the sample during irradiation. For instance, this phenomenon has been observed in Raman spectroscopy using the blue or green hnes of an argon ion laser which sometimes decompose sensitive sulfur samples with formation of Sg [2, 3]. Reliable spectra are obtained with the red hnes of a krypton ion or a He-Ne laser as well as with the infrared photons of a Nd YAG laser. 

The minimum energy needed to remove an electron from a potassium metal surface is 3.7 X 10 J. Will photons of frequencies 4.3 X 10 s (red light) and of 7.5 X 10 s (blue light) trigger the photoelectric effect If so, what is the maximum kinetic energy of the ejected electrons ... 

Importantly, the dead-time of TACs and TDCs is comparatively long, typically 125-350 ns. When a photon arrives within this time interval after the detection of a photon, it will not be observed. Therefore, care must be taken that the count rate of the experiment is sufficiently low to prevent this pulse-pileup. TACs and TDCs usually operate in reversed start-stop geometry. Here, the TAC is started by the fluorescence signal and stopped by the laser trigger. 

The active compound within the bacillary layer is retinal. To simplify the photo-physics within the rods and cones hugely, absorption of a photon initiates a series of conformational changes that lead ultimately to photo-isomerization of retinal from the 11-cis isomer to the 11-trans isomer see Figure 9.20. The uncoiling of the molecule following photo-excitation triggers a neural impulse, which is detected and deconvoluted by the brain. The photochemical reaction is breakage and, after rotation, re-formation of the C=C bond. 

From a different perspective, the lumophore appended receptor represents a molecular-scale light switch, which is triggered by a chemical species (whether or not it is of biological or technological consequence). Thus, photonic signals can be chemically generated with spatial, temporal, colour... 

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