Photo-cis-chlordane

Whether the isomers of chlordane, namely cis-chlordane and photo-cis-chlordane, were absorbed, retained, metabolized and excreted at similar or different rates was investigated by exposing goldfish or bluegill to 5 ppb concentration of each insecticide. 

Table 1. Bioaccumulation of cis-chlordane and photo-cis-chlordane by goldfish, bluegill and the amphibian Xenopus following their exposure to 5 ppb concentration in a static system.

Figure 2. Absorption of cyclodienes by fish and Xenopus in a static system (A), goldfish treated with photo-cis-chlordane (B), goldfish with cis-chlordane (C), blue gills with photodieldrin (D), blue gills with photo-cis-chlordane (E), bluegills with cis-chlorane and (F), Xenopus with ds-chlordane. All exposures were made at 5 ppb. (see Table 1 for biomass and volume of water).

Transfer of the aquatic animals, after absorption of the cyclodiene, to insecticide-free water showed variations in elimination pattern related with the chemical as well as its concentration in the body. It follows a somewhat biphasic response, there is initial rapid elimination of high concentration followed by a slow elimination at lower body concentrations (Fig. 3)(21,22, 23). Xenopus, even at low body levels of cis-chlordane, eliminate it at slightly faster rate than fish. Photo-cis-chlordane seems to be eliminated at a faster rate than cis-chlordane by both goldfish and bluegill (Table 2). The elimination of photo-cis-chlordane by goldfish and bluegill and of photodieldrin by blue-gill shows a biphasic response (Fig. 3). 

Feces from injected (7.9 mg/kg) male rats were collected for one week and extracted with acetone yielding about 50% of the dose. Fish were treated with 5 ppb of the compound in water for 48 hr and then held in clean water for 11 days at the end of which they were extracted whole with organic solvents. Recovery of the compound applied to fish was 74.3%. Chromatographs (0.25 mm Silica gel GF-254) were developed with heptane twice. The figure shows relative abundance of metabolites in the two species. PC is photo-cis-chlordane. 

Table 4. Analysis of metabolites of photo-cis-chlordane in blue-gill as analyzed by TLC (silica gel GF-254, 0.25 mm) followed by X-ray autoradiography and scintillation counting (16).

Under similar conditions bluegill absorb cis-chlordane at a higher rate than the amphibian, Xenopus laevis. Photo-cis-chlordane, an isomer of cis-chlordane is absorbed at a higher rate than cis-chlordane by bluegill and goldfish. However, photo-cis-chlordane is eliminated faster than cis-chlordane by the fish. 

Photolytic. Irradiation of cA-chlordane by a 450-W high-pressure mercury lamp gave photo-cis-chlordane (Ivie et al, 1972). 

CioHcCle, Photo-cis-chlordane, 45B, 172 CioHsClg, Photo-trans-chlordane, 45B, 172 CioHsCle, cis-Chlordane, 45B, 172 CioHsCler trans-Chlordane, 45B, 172... 

Chloranil, see Pentachlorophenol p-Choranil, see Pentachlorophenol Cis-Chlordan, see cfs-Chlordane a-Chlordan, see cfs-Chlordane p-Chlordane, frans-Chlordane y-Chlordane, see Heptachlor cfs-Chlordane, see Photo-cfs-chlordane frans-Chlordane, see Photo-frans-chlordane Chlordene, see Heptachlor... 

---