Phosphatidylcholines nuclear magnetic resonance

Kelusky, E. C. Smith, I. C. R, Anethetic-membrane interaction A 2H nuclear magnetic resonance study of the binding of specifically deuterated tetracaine and procaine to phosphatidylcholine, Can. J. Biochem. Cell Biol. 62, 178-184 (1984). 

There is abundant evidence to support the concept that the outer layer of plasma lipoproteins is a monolayer of polar lipids (phospholipids, mainly phosphatidylcholine, and cholesterol) and apolipoproteins with the hydrophilic aspect of the apolipoproteins and the polar head groups of phospholipids on the surface. The evidence has been reviewed by others [e.g., (S24)] and will not further be examined here. Nuclear magnetic resonance studies on HDL have shown that about 40% of unesterified cholesterol molecules are in the lipoprotein core, and 60% are associated with phospholipid molecules in the surface. Neither surface nor core is saturated with cholesterol (L20). Presumably, unesterified cholesterol is also found in the core of other lipoproteins. 

Vist, M. R. and Davis, J. H. (1990). Phase equilibria of cholesterol/dipalmitoyl-phosphatidylcholine mixtures H nuclear magnetic resonance and differential scanning calorimetry. Biochemistry 29 451. 

Holte LL, Peter SA, Sinnwell TM. Gawrisch K. -H nuclear magnetic resonance order parameter profiles suggest a change of molecular shape for phosphatidylcholines containing a polyunsaturated acyl chain. Biophys J 1995 68 2396-2403. 

Sears, B. (1975). /. Membrane Biol. 20, 59. 1 3C Nuclear Magnetic Resonance Studies of Egg Phosphatidylcholine. 

Hyphenated TLC techniques. TLC has been coupled with other instrumental techniques to aid in the detection, qualitative identification and, occasionally, quantitation of separated samples, and these include the coupling of TLC with high-pressure liquid chromatography (HPLC/TLC), with Fourier transform infra-red (TLC/FTIR), with mass spectrometry (TLC/ MS), with nuclear magnetic resonance (TLC/NMR) and with Raman spectroscopy (TLC/RS). These techniques have been extensively reviewed by Busch (1996) and by Somsen, Morden and Wilson (1995). The chemistry of oils and fats and their TLC separation has been so well established that they seldom necessitate the use of these coupling techniques for their identification, although these techniques have been used for phospholipid detection. Kushi and Handa (1985) have used TLC in combination with secondary ion mass spectrometry for the analysis of lipids. Fast atom bombardment (FAB) has been used to detect the molecular species of phosphatidylcholine on silica based on the molecular ion obtained by mass spectrometry (Busch et al, 1990). 

Han, X., Gross, R.W. (1991) Proton nuclear magnetic resonance studies on the molecular dynamics of plasmenylcholine/cholesterol and phosphatidylcholine/cholesterol bilayers. Biochim. Biophys. Acta 1063, 129-136. 

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