Phosphate buffer anions

The effect of concentration of cationic (cetylpyridinium chloride, CPC), anionic (sodium dodecylsulfate, SDS) and nonionic (Twin-80) surfactants as well as effect of pH value on the characteristics of TLC separ ation has been investigated. The best separ ation of three components has been achieved with 210 M CPC and LIO M Twin-80 solutions, at pH 7 (phosphate buffer). Individual solution of SDS didn t provide effective separation of caffeine, theophylline, theobromine, the rate of separ ation was low. The separ ation factor and rate of separ ation was increase by adding of modifiers - alcohol 1- propanol (6 % vol.) or 1-butanol (0.1 % vol.) in SDS solution. The optimal concentration of SDS is 210 M. 

Fig. 3. a) First order plot of oxygen uptake in the Methylene-blue (MB)-sensitized photooxidation of GA 8.4 pM and 1.3 mM histidine (control) in phosphate buffer pH 7. b) Percentage radical scavenging activity for the control molecule Trolox and GA at pH 7.4 in phosphate buffer 10 mM (hydroxyl radical) and pH 10 in sodium carbonate buffer 50 mM (anion superoxide radical). 

Fig. 5.38 Reduction of 10-3m phenylglyoxylic acid at the mercury streaming electrode in acetate and phosphate buffers containing 1 m KN03 (1) pH 5.02, (2) pH 5.45, (3) pH 5.85, (4) pH 6.25. The curves 2, 3 and 4 are shifted by 0.2 V, 0.4 V and 0.6 V with respect to curve 1. The first wave is controlled by the surface protonation reaction while the second is a direct reduction of the acid anion. (According to J. Koryta)...

Figure 8 Anion exchange separation of diethanolamine (DEA), glycine, hydroxyethyl glycine (HEG), iminodiacetic acid (IDA), and glyphosate followed by ECL detection. Mobile phase consisting of 0.01 mM Ru(bpy)32+ in 10% acetonitrile, 90% 0.01 M phosphate buffer at pH 9.8. (Reprinted from Ref. 61, with permission from, and copyright, Elsevier Science.)...

It is noteworthy that in om NMR experiment 0.1 M sodium phosphate buffer remarkably attenuated the interaction between cardiolipin and cytochrome c, indicating that electrostatic interactions between anionic... 

Figure 6 is an example of organic acids and anions analyzed with a 70 mM phosphate buffer at pH 2.5 and 200 nm. 

FIGURE 6 Analysis of UV-absorbing anions and organic acid is possible with a 70mM phosphate buffer at pH 2.5. 

Chromatospac Prep 10 silica gel, 2-propanol-ethyl acetate-HjO strong anion-exchange silica-gel columns ( 1 x 50 cm), phosphate buffers... 

It should be emphasized that the results presented in this paper were obtained only in 0.1M HClOj and phosphate buffer (pH=5.6). Work currently in progress indicates that anions in the electrolyte play a role in the electrochemical processes leading to inhibition. The effectiveness of the Inhibitors has been found to vary depending on the testing solution. 

Figure 2. Anion exchange chromatogram of ionically bound phloem peroxidases on DEAE-Sepharose. Collected fractions were analyzed for their oxidase activity towards TMB (1), syringaldazine (2) and isopropylamine salt from / -fluoroferulic acid (3). Bo cationic peroxidases Bj and B2 anionic peroxidases. Column was equilibrated with 0.01 M phosphate buffer (pH 7.1). Fractions were eluted with a NaCl gradient (0-0.5 M) in the same buffer (0.01 M phosphate, pH 7.1).

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