Phosphatases families

LMWPc Low molecular weight phosphatase family E(MF)AB 1(1) 0(0) 1BVH... 

PP2Cc Serine/threonine phosphatases, family 2C, catalytic domain E(MFP)B 8(8) 11(11) 1A6Q... 

Gary, J.D. Sato, T.K. Stefan, C.J. Bonangelino, C.J. Weisman, L.S. Emr, S.D. Regulation of fabl phosphatidylinositol 3-phosphate 5-kinase pathway by vac7 protein and fig4, a polyphosphoinositide phosphatase family member. Mol. Biol. Cell, 13, 1238-1251 (2002)... 

In general there are three phosphatase families alkaline, acid, and protein phosphatases. Alkaline phosphatases are typically dimers that contain three metal ions per subunit and have a pH optimum pH above 8. Acid phosphatases exhibit an optimum pH<7 and are usually divided into three classes low molecular weight acid phosphatases (<20 kDa), high molecular weight acid phosphatases (50-60 kDa), and purple acid phosphatases (which contain an Fe-Fe or Fe-Zn center at the active site). Phosphatases specific for I-l-P appear to be most similar (in kinetic characteristics but not in mechanism) to the alkaline phosphatases, but their structures define a superfamily that also includes inositol polyphosphate 1-phosphatase, fructose 1, 6-bisphosphatase, and Hal2. The members of this superfamily share a common structural core of 5 a-helices and 11 (3-strands. Many are Li+-sensitive (York et al., 1995), and more recent structures of archaeal IMPase proteins suggest the Li+ -sensitivity is related to the disposition of a flexible loop near the active site (Stieglitz et al., 2002). 

The glucose-l-phosphatase from E. coli hydrolyzes phytate as well as glucose-1-phosphate. This enzyme is a member of the histidine acid phosphatase family (Lee et al., 2003). Glucose-l-phosphatase cleaves only the 3-phosphate from phytate, and no further hydrolysis takes place. It is the only phosphatase... 

Chi, H., Tiller, G.E., Dasouki, M.J., Romano, P.R., Wang, J., O Keefe, R.J., Puzas, J.E., Rosier, R.N., and Reynolds, P.R., 1999, Multiple inositol polyphosphate phosphatase evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10-23 and 19. Genomics 56 324-336. 

Class 111 Hydrolase Phosphatase Family E. coli lALK Znl 4.0 Asp 3 HiSacK 80 His H2O ... 

Purple acid phosphatase Family Kidney bean IKBP Fe(lll) Zn 3.3... 

The alkaline phosphatase family contains three metals in the active site while the rest of the cocatalytic zinc sites contain two metals (Figme 10) (Table 3). Some of these sites contain metals such as copper, iron, and magnesium in combination with zinc. Combinations of Cu(II)/Zn are seen in the superoxide dismutase (SOD) family see Copper Proteins with Type 2 Sites), Zn/Mg are seen in alkaline phosphatase family and lens aminopeptidase, and Fe(III)/Zn in the purple acid phosphatase family. 

Laporte J, Hu LJ, Kretz C et al (1996) A gene mutated in X-hnked myotubular myopathy defines a new putative tyrosine phosphatase family conserved in yeast. Nat Genet 13 ... 

Jacobson, R.S., and Hoffman, D.R., 1995, Honey-bee acid phosphatase is a member of the prostatic acid phosphatase family. J. Allergy Clin. Immunol. 95 372. (abstract)... 

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