PHD fingers

Found in the transcriptional repressor NF-Xl, a PHD finger/RING finger protein. 

ScHEEL, H. and K. Hoemann, No evidence for PHD fingers as ubiquitin ligases. Trends Cell Biol, 2003, 13(6), 285-7 author reply 287-8. 

Bordoli L, Husser S, Luthi U, Netsch M, Osmani H, Eckner R (2001a) Functional analysis of the p300 acetyltransferase domain the PHD finger of p300 but not of CBP is dispensable for enzymatic activity. Nucleic Acids Res 29 4462 471... 

Kalkhoven E (2004) CBP and p300 HATS for different occasions. Biochem Pharmacol 68(6) 1145-1155 Kalkhoven E, Roelfsema JH, Teunissen H, den Boer A, Ariyurek Y, Zantema A, Breuning MH, Hennekam RC, Peters DJ (2003) Loss of CBP acetyltransferase activity by PHD finger mutations in Rubinstein-Taybi syndrome. Hum Mol Genet 12(4) 441 50... 

With regard to specificity, plant homeodomain (PHD) fingers constitute the most diverse class of histone binding modules. These modules recognize lysine in unmodified, mono-, di- and trimethylated states. Although not stmcturally related. 

Figure 1.2 Structural comparison of (a) the E237 and D230. In the RAG2-PHD finger ING2 [50] (PDB entry 2C6Q) and (b) the structure, Me3l<4 is enclosed in an aromatic...

In this cooperative binding event, the Rcol-PHD finger is primarily responsible for providing affinity for nucleosomes, while the Eafi chromodomain contributes specificity for the methylated H3K36 mark [59, 60]. These examples demonstrate how multiple domains can cooperate to read a histone code with high fidelity and enhanced affinity. 

Matthews, A.G. et al. (2007) RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. Nature, 450, 1106-1110. 

Org, T. et al. (2008) Peterson, The autoimmune regulator PHD finger binds to non-methylated histone H3K4 to activate gene expression. EM BO Reports, 9, 370-376. 

Li, H., llin, S., Wang, W Duncan, E.M., Wysocka, J., Allis, C.D. and Patel, D.J. (2006) Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF. Nature,... 

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