Phage antibodies selection from libraries

The affinity of antibodies selected from naive, synthetic, or even immune phage libraries is typically sufficient for use as a research reagent, but too low for some specific therapeutic applications such as viral neutralization or tumor imaging. For many applications, affinity maturation can be bypassed completely by the construction of multivalent molecules. If this is not sufficient selected antibody clones may be affinity matured (see Figure 18.13). 

Shadidi, M. and M. Sioud (2001). An anti-leukemic single chain Fv antibody selected from a synthetic human phage antibody library. Biochem Biophys Res Commun 280(2) 548-552. 

De novo antibody selection from very large and diverse phage libraries In vivo technologies Useful for natural or anti-pathogen antibodies, can be combined with phage display... 

Figure 16.4 Graph depicting the percentage of lysine residues among peptides that bind to the indicated monoclonal antibodies. The peptides were isolated after affinity selection (biopanning) from a phage-displayed combinatorial peptide library. The peptides are grouped as to whether they are susceptible to formalin fixation, resulting in a loss of immunoreactivity.

Willems, P.M., Hoet, R.M., Huys, E.L., Raats, J.M., Mensink, E.J., Raymakers, R.A. (1998). Specific detection of myeloma plasma cells using anti-idiotypic single chain antibody fragments selected from a phage display library. Leukemia, 12, 1295-1302. 

De Kruif, J., Terstappen, L, Boel, E, and Logtenberg, T (1995) Rapid selection of cell subpopulation-specific human monoclonal antibodies from a synthetic phage antibody library Proc Natl Acad Sci. USA 92, 3938—3942... 

Making and Selecting from Phage Antibody Libraries... 

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