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Peroxisomes protein import, permeabilized cell system

A Permeabilized Cell System to Study Peroxisomal Protein Import... [Pg.140]

The development of permeabilized cell systems has facilitated the reconstitution and biochemical characterization of a variety of intracellular protein trafficking steps. In this article I describe how coverslip-attached cells are permeabilized with streptolysin O (SLO) and incubated with exogenously added peroxisomal proteins. The protein import into peroxisomes is analyzed by a simple immunofluorescence technique. The procedure is described for CHO cells but it can be used for a variety of mammalian cells. Furthermore, the system can be applied to study the import of proteins into other subcellular organelles. [Pg.140]

Wendland, M., and Subramani, S. (1993a) Cytosol-dependent peroxisomal protein import in a permeabilized cell system. J. Cell Biol. 120, 675-685. [Pg.147]

See other pages where Peroxisomes protein import, permeabilized cell system is mentioned: [Pg.146]   
See also in sourсe #XX -- [ Pg.3 , Pg.140 , Pg.141 , Pg.142 , Pg.143 , Pg.144 , Pg.145 , Pg.146 ]



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Cell permeabilization

Important Proteins

Permeabilization

Permeabilized cells

Permeabilizing

Peroxisomes

Protein import

Protein importance

Protein system

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