Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Permeabilization with streptolysin

Ahnert-Hilger G, Bader MF, BhakDai S et al. (1989b) Introduction of macromolecules into bovine adrenal medullary chromaffin cells and rat pheochromocy-toma cells (PC12) by permeabilization with streptolysin O inhibitory effect of tetanus toxin on catecholamine secretion. J. Neurochem. 52 1751 -8... [Pg.211]

The development of permeabilized cell systems has facilitated the reconstitution and biochemical characterization of a variety of intracellular protein trafficking steps. In this article I describe how coverslip-attached cells are permeabilized with streptolysin O (SLO) and incubated with exogenously added peroxisomal proteins. The protein import into peroxisomes is analyzed by a simple immunofluorescence technique. The procedure is described for CHO cells but it can be used for a variety of mammalian cells. Furthermore, the system can be applied to study the import of proteins into other subcellular organelles. [Pg.140]

See other pages where Permeabilization with streptolysin is mentioned: [Pg.187]    [Pg.187]    [Pg.252]    [Pg.199]    [Pg.260]    [Pg.357]    [Pg.145]    [Pg.77]    [Pg.83]   
See also in sourсe #XX -- [ Pg.3 , Pg.142 ]



SEARCH



Permeabilization

Permeabilization Streptolysin

Permeabilizing

Streptolysin

© 2024 chempedia.info