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Intracellular protein trafficking

Amara, J.F., Cheng, S.H. and Smith, A.E. (1992) Intracellular protein trafficking defects in human disease. Trends Cell Biol. 2, 145-150. [Pg.409]

Many of the components required for intracellular protein trafficking have been Identified in yeast by analysis... [Pg.707]

The development of permeabilized cell systems has facilitated the reconstitution and biochemical characterization of a variety of intracellular protein trafficking steps. In this article I describe how coverslip-attached cells are permeabilized with streptolysin O (SLO) and incubated with exogenously added peroxisomal proteins. The protein import into peroxisomes is analyzed by a simple immunofluorescence technique. The procedure is described for CHO cells but it can be used for a variety of mammalian cells. Furthermore, the system can be applied to study the import of proteins into other subcellular organelles. [Pg.140]

Protein Trafficking and Quality Control Intracellular Transport Palmitoylation Endothelins... [Pg.223]


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Intracellular proteins

Protein trafficking

Trafficking

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