Peroxides, detection thin layer chromatography

Thin-Layer Chromatography. Thin-layer chromatography was used for many of the compounds which are of interest for either qualitative or quantative analysis. These techniques were used primarily as screening methods to supplement other analytical methods. For some brominated compounds such as TRIS, the detection system reported by Hahn (9) was applicable. This method is based upon the reaction of fluorescein with elemental bromine released through peroxide oxidation. An evaluation in our laboratory of this detection system indicated that semi-quantitative results... 

As peroxy compounds differ from one another in solubility, it is obvious that several solvent systems are necessary for their separation. The following solvent systems have been found suitable (49—51) dimethylformamide/ /hexane to benzene ethylene glycol/hexane formamide/hexane -butanol-ethanol-water (45 5 50) or (10 10 1). For detection, sensitive down to 1 pg, the following sprays have been recommended (a) 0.1% p-amino-dimethylaniline hydrochloride and (b) a mixture of 3 ml of glacial acetic acid, 2 ml of a saturated potassium iodide solution, and 5 ml of starch solution. For the chromatography of organic peroxides on thin layers see (52, 53). 

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