PEG-amine

Hong et al. (2004) also found that modification of PAMAM dendrimers with a short PEG linker arm could act to reduce nonspecificity caused by the amines on the dendrimer-modified surface. An azido-PEGj-aininc spacer was activated with nitrophenyl carbamate to yield an activated intermediate that could be used to modify the amines on the dendrimer (Figure 7.24). Reaction at high molar ratio resulted in about 61 PEG-azido spacers on the dendrimer. Reduction of the azido group to an amine using triphenylphosphine in THF provided the dendrimer-PEG-amine derivative for surface modification. The added presence of the PEG spacer arm reduced... 

Dissolve an amine-containing ligand in 0.1 M sodium carbonate, pFI 9.5. If coupling a small ligand, such as a biotin-PEG-amine compound (Chapter 18), then use a concentration of about 5-10 mM in the carbonate buffer. For proteins, concentrations of 10 nM to 1 pM can be used with success. 

Figure 18.21 Biotin-PEG -amine compounds can be used to modify carboxylate- or aldehyde-containing compounds using a carbodiimide reaction.

Figure 18.22 Biotin-PEG -amine can be used to add a biotin label to carboxylate-containing molecules using the EDC/(sulfo)NHS reaction, which forms a stable amide linkage.

Dissolve the biotin-PEG -amine reagent in reaction buffer at a concentration of 25 mM. 

Add a quantity of the biotin-PEG -amine solution to the solution containing the car-boxylate molecule to achieve the desired molar excess. For molecules containing a single carboxylate to be modified, a 1.5- to 2-fold molar excess may be sufficient. However, for proteins or peptides that also contain competing amines, a much larger excess of biotin compound should be used (e.g., 100-fold excess). For instance, for protein biotinylation, add 120 pi of the biotin-PEG -amine solution per ml of the solution prepared in Step 1. 

Figure 25.10 PEG-amine compounds may be reacted with this heterobifunctional crosslinker to form amide bond derivatives terminating in maleimide groups. This results in a homobifunctional reagent capable of crosslinking thiol molecules. Subsequent reaction with sulfhydryl-containing molecules yields thioether linkages.

PEG-Amine Derivative (Jeffamine Series from Texaco Various Polymer Lengths Available)... 

Joon et al. (282) recently reported the synthesis of a 10-member array of 3-aminoimi-dazoline-2,4-diones L24 (Eig. 8.48) using MeO-PEG-amine soluble support, the aromatic linker 8.98, and the monomer sets Mi (six a-amino acid isocyanates) (283) and M2 (four aza a-amino acids) (247). The linker was coupled to the support via an amide bond (step a, Eig. 8.48), and deprotection of the phenolic hydroxyl (step b) gave supported 8.99. Coupling with monomer set Mi (step c) and removal of the butyl ester (step b) produced 8.100, which was coupled to monomer set M2 (step d) and deprotected (step b) to give 8.101. Each deprotection step was followed by precipita-... 

Applications with sodium cyanoborohydride now include the synthesis of large molecular entities used in biological testing. Conjugation of lactose to human serum albumin by reductive amination (eq 37) and carbohydrate conjugation with PEG amines have been added to the array of transformations reported for NaBHsCN. 

Synonyms Bis-2-hydroxyethyl oleamine Bis (2-hydroxyethyl) oleylamine N,N-Di (2-hydroxyethyl) oleylamine PEG-2 oleyl amine PEG 100 oleyl amine POE (2) oleyl amine Definition PEG amine of oleic acid Empirical CjjHkNOj... 

Synonyms POE (20) oleyl amine Definition PEG amine of oleic acid... 

Synonyms Bis (2-hydroxyethyl) tallowamine POE (2) tallowamine Definition PEG amine of tallow Empirical C22H N02... 

CAS 61790-82-7 (generic) 61791-44-4 (generic) 90367-28-5 (generic) Synonyms POE (20) tallowamine Definition PEG amine of tallow... 

Definition PEG amine of hydrogenated tallow Formuia R-N(CH2CH20)xH(CH2CH20)yH, R rep. alkyl groups from hydrog. tallow, avg. (x+y) = 14 Uses Antistat, dyeing assistant, softener, dispersant in textiles... 

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