PCI 2 cells

In several studies, chronic ethanol treatment has been associated with PKC upregulation. In PCI 2 cells, increased levels of PKCg and e were found (25-200 mM ethanol, 2-8 days of treatment) which was associated with increased PKC-mediated phosphorylation. 

Borgatti P, Mazzoni M, Carini C, Neri LM, Marchisio M, Bertolaso L, Previati M, Zauli G, Capitani S 996) Changes of nuclear protein kinase C activity and isotype composition in PCI 2 cell proliferation and differentiation. Exp Cell Res 224 72-78... 

Vimentin is not the only IF type that is moved in this manner. Neural IFs, including both the type III peripherin proteins and type IV neural IF proteins, have also been demonstrated to be moved by molecular motors. In the case of peripherin, particles and squiggles were observed to translocate rapidly within PCI 2 cell bodies, neurites, and growth cones. The movements were bidirectional and dependent on microtubules, kinesin, and cytoplasmic dynein. The authors suggest that peripherin particles are... 

Mee CJ, Tomlinson SR, Perestenko PV et al (2004) Latrophilin is required for toxicity of black widow spider venom in Caenorhabditis elegans. Biochem J 378 185-91 Meiri H, Erulkar SD, Lerman T et al (1981) The action of the sodium ionophore, monensin, or transmitter release at the frog neuromuscular junction. Brain Res 204 204-8 Meldolesi J, Huttner WB, Tsien RY et al (1984) Free cytoplasmic Ca2+ and neurotransmitter release studies on PCI 2 cells and synaptosomes exposed to a-latrotoxin. Proc Natl Acad Sci US A 81 6204... 

Salinas, M., Lopez-Valdahso, R., Martin, D., Alvarez, A., and Cuadrado, A., 2000, Inhibition ofPKB/Aktl by C2-ceramide involves activation of ceramide-activated protein phosphatase in PCI 2 cells. Mol. Cell. Neurosci. 15 156-169. 

Edsall, L.C., Cuvillier, O., Twitty, S., Spiegel, S. and Milstien, S., 2001, Sphingosine kinase expression regulates apoptosis and caspase activation in PCI 2 cells, J. Neurochem. 76 1573-1584. 

Satpute, R.M., Hariharakrishnan, J., Bhattacharya, R. (2008). Alpha-ketoglutarate and V-acetyl cysteine protect PCI 2 cells from cyanide-induced cytotoxicity and altered energy metabolism. Neurotoxicology 29 170-8. 

A detailed characterization of HENECA in comparison with CGS21680, NECA, and CCPA is reported in Table 2. In this Table, in addition to the binding data, the activity on the stimulation of adenylate cyclase in platelet and PCI 2 cells is shown. The results of the cyclase assays are in good agreement with the higher affinity of HENECA for the A2a receptor in comparison with the other adenosine agonists [22]. 

Fig. 7.1-7 Reduction of PCI 2 cell differentiation rate by Raspalin in the PCI 2 differentiation assay.

Jonnala, R. R., and Buccafusco. J. J, (1998), Nicotine increases the cxpre.ssion of TrkA receptors on differentiated PCI 2 cells. Soc. Neum.sci. Ahstr. 24, 1340. 

FIGURE20.12 Separation of dopamine (DA) and catechol (CAT) from a single mammalian cell, (a) Dopamine was separated and detected in a 380 fL injection of PCI 2 cell cytoplasm and (b) a standard injection of DA and CAT is used to verify the detection of dopamine. Both injections used a 3 kV injection voltage for 5 s and a bare fused-silica capillary. Dopamine was separated at 25 kV in 50 mM TES, pH 7.2 with 2% 1-propanol. (Reprinted from Woods, L. A., et al., Electroanalysis, 17,1192, 2005. With permission.)... 

Fig. 1. ADP-ribosylation of PC12 cell lysates by Clostridium botulinum culture supernatant. Portions of lysates were P pjADP-ribosylated without (lane 1) or with (lane 2) a preceding incubation with unlabeled NAD and toxin. Both samples contained 20 pM unlabeled NAD and 100 p ml supernatant protein. 5 pM [32p]NAD was added to the mixture in sample 1 immediately and to sample 2 after first preincubating for 30 min at 37 C. Both samples were incubated with p P]NAD for 30 min at 37 C. Culture supernatant of C. botulinum strain 17784 was partially purified by precipitation with ammonium sulfate and acid (5). PCI 2 cells (12) were pelleted by centrifugation and were lysed by freezing and thawing. Labeling was performed using ca. 50 pg of cellular protein and 5pM [32p]NAD (250,000 c.p.m.) Samples were incubated for 30 min at 37°C and were then precipitated with 10% cold trichloroacetic acid. Precipitates were dissolved, neutralized and analyzed by SDS-PAGE and autoradiography. For quantitation, bands were cut out of dried gels and counted. The uppermost radioactive band represents poly(ADP-ribose) polymerase which is labeled independently of the toxin.

Electrochemical Monitoring of Exocytosis from Individual PCI 2 Cells 311... 

Study of Exocytosis with an Artificial Cell Model Constructed FROM PCI 2 Cell Plasma Membrane Vesicles (Blebs)... 

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