Pasteurella multocida sialidase

Another multifunctional bacterial enzyme that has been reported recently is Pasteurella multocida sialyltransferase (PmSTl or tPm0188Ph) (67, 68). It has four different activities, including an a2,3SiaT, a2,6SiaT, a2,3-sialidase, and trans-sialidase activities. PmSTl is a highly active sialyltransferase with broad donor and acceptor substrate specificity therefore it is a powerful tool... 

Our group has reported a novel multifunctional bacterial enzyme, Pasteurella multocida sialyltransferase (PmSTl). PmSTl is an easy to express extremely soluble, and highly active sialyltransferase. Routinely, about 100 mg of PmSTl can be purified from one liter E. coli cell culture. PmSTl has four different activities including a2,3-sialyltransferase (optimal activity at pH 7.5-9.0), a2,6-sialyltransferase (optimal activity at pH 4.5-7.0), a2,3-sialidase (optimal activity at pH 5.0-5.5), and trans-sialidase (optimal activity at pH 5.5-6.5) activities. Among them, a2,3-sialyltransferase activity is the major function of the PmSTl with a specific activity of 60 U/mg protein (24),... 

Aldolases, CMP-Sia synthetases, and/or sialyltransferases have been combined in a number of one-pot, chemoenzymatic syntheses of naturally occurring sialosides (e.g., see Ichikawa et /. ). The use of permissive enzymes broadens the scope of these one-pot methods and has facilitated the synthesis of a number of unnatural sialosides. The Chen group reported a Pasteurella multocida sialyltransferase that can function as an a2-3 sialyltransferase, o 2-6 sialyltransferase, o 2-3 sialidase, or a2-3 rr j-sialidase, depending on the pH of the reaction mixture. Using this enzyme in a one-pot reaction in combination with E. coli aldolase and... 

Corynebacterium diphtheria,Campylobacter jejuni, and Pasteurella multo-cida Remarkably, the C. jejuni TS exemplifies an oligosaccharide lians-sialidase that specifieally transfers (or hydrolyzes) a-(2— 8)-linkages of siahc acid. The P. multocida enzyme exhibits a-(2—>3) TS activity. Even more striking is the fact drat the latter two baeterial enzymes possess, besides their TS and sialidase activities, additional elassieal sialylliansferase activities with CMP-Neu5Ac as donor. 

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