Partition chromatography precoated layers

Chapter 3 through Chapter 8 deal with the basic aspects of the practical uses of PLC. Chapter 3 describes sorbent materials and precoated layers for normal or straight phase (adsorption) chromatography (silica gel and aluminum oxide 60) and partition chromatography (silica gel, aluminum oxide 150, and cellulose), and precoated layers for reversed-phase chromatography (RP-18 or C-18). Properties of the bulk sorbents and precoated layers, a survey of commercial products, and examples of substance classes that can be separated are given. 

Sorbent Materials and Precoated Layers for Partition Chromatography.54... 

SORBENT MATERIALS AND PRECOATED LAYERS FOR PARTITION CHROMATOGRAPHY... 

Precoated plates are also available for reversed-phase liquid-liquid partition thin-layer chromatography. Here the silica gel has been treated with an octadecyl silylating reagent thus coating the particles with a non-polar chemically-bonded thin film. The solvent employed is more polar than the film and chromatographic development results from partition between these two phases. 

Precoated TLC plates with both native and microcrystalline cellulose layers have been used for the separation of carbohydrates and other very polar compounds for several years (3), Table 1. The main advantage of these low surface area sorbents is that most of the separations previously achieved on paper can be obtained by TLC using the same solvent systems as in paper chromatography (3). It is generally assumed that a partition mechanism is responsible for retention on these materials. Cellulose... 

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