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Particles glutaraldehyde coupling

After the final wash, suspend the particles in coupling buffer containing 10 percent glutaraldehyde and mix well to dissolve. [Pg.602]

Wash the particles with coupling buffer at least several times using centrifugation to remove excess glutaraldehyde. Resuspend in 1 ml of the same buffer. [Pg.602]

The two-step glutaraldehyde coupling reaction has the advantage over the one-step reaction and the carbodiimide coupling reaction in that intramolecular and intermolecular crosslinking of the antibody molecules is avoided. Furthermore, uinder these conditions, aggregation of the latex particles does not occur. [Pg.245]

Choline oxidase has been immobilized, using a variety of procedures, on hydrophobic agarose particles (Karube et al., 1979c), asymmetric acetylcellulose membranes (Mizutani and Tsuda, 1982), and nylon netting (Mascini and Moscone, 1986). Yao (1983) constructed a CME by direct fixation of choline oxidase on a silanized platinum net electrode via glutaraldehyde coupling. [Pg.207]

Adsorption onto tannin coupled to aminohexyl-cellulose Glutaraldehyde-mediated reaction with sub-micron ferrite particles... [Pg.687]

See other pages where Particles glutaraldehyde coupling is mentioned: [Pg.173]    [Pg.601]    [Pg.601]    [Pg.602]    [Pg.602]    [Pg.321]    [Pg.204]    [Pg.241]    [Pg.242]    [Pg.164]    [Pg.165]    [Pg.31]    [Pg.173]    [Pg.108]    [Pg.88]    [Pg.93]    [Pg.378]    [Pg.300]   
See also in sourсe #XX -- [ Pg.602 ]



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