Panning procedure

The screening or panning procedure can also be carried out using cell lines expressing the antigen of interest either in a tissue-culture plate-based approach... 

Coat ELISA plates as for the panning procedure, and block with 200 pL PBS containing 1 % BSA for 1 h... 

Cells are seeded onto tissue culture wells 24-48 h before panning. Only a single well is required for each panning round. Once started, each round of the panning procedure requires approximately 4-5 h to complete. Additional time is required for bacterial plating for titer determination and phage amplification. 

The panning procedure requires approximately 4h to complete. The cells can be removed directly from a feeder flask for the panning procedure. They do not have to be seeded into a separate flask prior to the day of the panning procedure. 

Fig. 11 Panning procedures the chromatopanning protocol without top) and with (bottom) on-column infection. (Reproduced from [63] with permission)...

Development of extraction-free photometric procedures for the determination of traces of metals for which hygienic and environmental regulations have been established is an urgent problem. For solution of this problem we used as an organic reagent l-(2- pyridylazo)-naphtol-2 (PAN) which forms intensely coloured complex compounds with many metals and is frequently used for their extraction-photometric determination however these procedures did not find wide application in water analysis due to lack of selectivity and necessity of using organic solvents. 

The lattice-type shelf is functionally as good as the others, but it may not look appropriate for a book shelf in the context of a library. A second consideration is a combination of physical requirements and appearance. A simple plastic beam that will function adequately in terms of strength and stiffness may be rather thin. A shelf of this type can look flimsy even if it is functional. This impression is useful to the designer since the solid plate is probably an uneconomical use of material. A requirement was added that the design should look like a wood shelf since this is the context in which it is to be used. To produce the desired thickness appearance either a lipped pan with internal reinforcement can be used or, alternatively, a sandwich-type structure with two skins and a separator core. In either case the displacement of the material from the plane of bending will improve the stiffness efficiency of the product. The appropriate procedure is to... 

Compared to my other laboratory experiences, 1 would say that this lab was by far the most difficult and time consuming, but probably the most educational. 1 never fully appreciated the effort that it took to come up with one s own procedure when the prescribed procedure did not pan out. ... 

A typical testing procedure involves several steps. First, the selected number and size of sieves are stacked upon one another, with the largest openings (inversely related to mesh per inch) being at the top of the stack, and beneath that a pan to collect the particles finer than the smallest sieve. The known amount of powder to be analyzed is then placed on the top sieve and the set is vibrated in a mechanical device for a predetermined time period. The results are obtained by weighing the amount of material retained on each sieve and on the collecting pan. The suction method uses one sieve at a time and examines the amount retained on the screen. In both methods the data are expressed as frequency or cumulative frequency plots, respectively. 

The above test-design and scale-up procedures have been applied to numerous long-distance systems (e.g., fly ash, pulverized coal) and have been found to provide good accuracy and reliability. Some examples of these results have been presented by Pan and Wypych (1992a) and Pan and Wypych (1994). The interested reader is referred to these papers for further details. The above procedures also have been used successfully to... 

If accurate design/analysis data are necessary (e.g., Vjmm, Vfup), it is possible to obtain the information needed by undertaking the appropriate bench-type tests and large-scale test-design procedures, similar to the approaches employed for pneumatic conveying (Pan and Wypych, 1992a Arnold et al., 1994). 

By employing accurate test-design procedures (Pan and Wypych, 1992a), it is possible to model and design each pipe branch separately so that the system ultimately is well balanced. However, such a system may not be reliable over time due to uneven wear in the pipes/bends, changes in material property and/or on-site conditions. 

Pan, R., and Wypych, P. W., Scale-Up Procedures for Pneumatic Conveying Design, Powder Handling Processing, 4(2) 167—172 (1992a)... 

A completely different approach was taken by Koresh and Soffer (1980, 1986, 1987). Their preparation procedure involves a polymeric system like polyacrylonitrile (PAN) in a certain configuration (e.g. hollow fiber). The system is then pyrolyzed in an inert atmosphere and a dense membrane is obtained. An oxidation treatment is then necessary to create an open pore structure. Depending on the oxidation treatment typical molecules can be adsorbed and transported through the system. 

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