P IODOPHENOL

C36H60O30 CeHsIO 3 H20 Cyclomaltohexaose - p-iodophenol, trihydrate (CHAIPL)276... 

P212121 Z = 4 Dx = 1.52 R = 0.07 for 4,811 intensities. This structure is isomorphous with that of the p-hydroxybenzoic acid,278 p-iodophenol,276 and p-iodoaniline277 complexes. 

C42H70O35 3 C8HsIO-24 H20 Cyclomaltoheptaose-p-iodophenol, tetracosahydrate (BCDIPH10)332... 

C42H70O35 3 C6H5IO 24 H20 Cyclomaltoheptaose - p-iodophenol, tetracosahydrate BCDIPH10 43 372... 

CL reaction can be catalyzed by enzymes other than HRP (e.g., microperoxidase and catalase) and by other substances [hemoglobin, cytochrome c, Fe(III), and other metal complexes]. The presence of suitable molecules such as phenols (p-iodophenol), naphthols (l-bromo-2-naphthol), or amines (p-anisidine) increases the light production deriving from the HRP-catalyzed oxidation of luminol and produces glow-type kinetics [6, 7], The use of other enzymes, such as glucose-6-phosphate dehydrogenase [38-41], P-galactosidase [42], and xanthine oxidase [43-46], as CL labels has been reported. 

Iodoform, 297, 299 test, 1068, 1069 p-Iodonitrobenzene, 600 2-Iodopentane, 288 p-Iodophenol, 666, 680 Iodosobenzene, 534, 541 p-Iodotoluene, 591, 599 Iodoxybenzene, 534, 542 Ion-exchange resins, 1019 see Anion exchange - resin and Cation exchange resin... 

Working enhanced chemiluminescence substrate solution (see Note 9)—either a. /Modophenol-enhanced substrate luminol 1.25 mM, p-iodophenol 4 pM,... 

H2Oz 2.7 mM). Add 15 pL of stock hydrogen peroxide and 40 mL of p-iodophenol stock solution to 50 mL of stock substrate solution, and mix well. Make up daily using high-quality deionized water (see Note 3), and store in the dark when not in use, or... 

The p-iodophenol solidifies upon cooling. The yield of material melting at 32-340 is 70 g. (63 per cent of the theoretical amount). The product obtained in this way is slightly yellow. If a purer product is desired, it may be redistilled in vacuo. 

HRP-based Luminol/H202/ p-iodophenol Oxidized luminol substrate gives off blue light p-iodophenol increases light output Very convenient, sensitive system reaction is detected within a few seconds to 1 hr... 

Removal rates greater than 90% were observed for all of the phenols studied. The decay kinetics for phenol p-methoxyphenol p-cresol p-fluo-rophenol p-chlorophenol p-bromophenol 4-hydroxyacetophenone a,a,a-trifluoro-p-cresol p-cyanophenol and p-iodophenol were found to be consistent with the Langmuir-Hinshelwood kinetic model. After employing all the substituents, little variation on the Langmuir-Hinshelwood kinetic parameters was observed. 

Chemiluminescence of oxidized luminol has been the basis of several lumino-metric methods of estimation of TAC (Table 1). The mostcommon is to measure the induction time of the reaction. Often the chemiluminescence is first induced by an oxidant and then attenuated by addition of a sample, and the time to recover the initial fluorescence is measured. The enhanced chemiluminescent assay introduced a decade ago is based on the oxidation of luminol by perborate or by hydrogen peroxide in a reaction catalyzed by horseradish peroxidase. Enhancement (and stabilization) of luminescence is achieved by addition of p-iodophenol. The original procedure used a commercial reagent kit (ECL Anti-oxidant Detection Pack... 

---