P450 fusion protein

Rodrigues, A.D. Mulford, D.J. Lee, R.D. Surber, B.W. Kukulka, M.J. Ferrero, J.L. Thomas, S.B. Shet, M.S. Estabrook, R.W. In vitro metabolism of terfenadine by a purified recombinant fusion protein containing cytochrome P4503A4 and NADPH-P450 reductase. Comparison to human liver microsomes and precision-cut liver tissue slices. Drug Metab.Dispos., 1995, 23, 765-775... 

Fisher, C.W., M.S. Shet, D.L. Caudle, C.A. Martin-Wixtrom, and R.W. Estabrook (1992). High-level expression in Escherichia coli of enzymatically active fusion proteins containing the domains of mammalian cytochromes P450 and NADPH-P450 reductase flavoprotein. Proc. Natl. Acad. Sci. USA 89, 10817-10821. 

Chun, Y.J., X. Shimada, and F.P. Guengerich (1996). Construction of a human cytochrome P450 lAl Rat NADPH-cytochrome P450 reductase fusion protein cDNA and expression in Escherichia coli, purification, and catalytic properties of the enzyme in baeterial eells and after purification. Arch. Biochem. Biophys. 330,48—58. 

Shet, M., C.W. Fisher, P.L. Holmans, and R.W. Estabrook (1996). Xhe omega-hydroxlyation of lau-ric acid Oxidation of 12-hydroxylauric acid to dodecanedioic acid by a purified reeombinant fusion protein containing P450 4A1 and NADPH-P450 reductase. Arch. Biochem. Biophys. 330,199-208. 

Deeni, Y.Y., M.J. Paine, A.D. Ayrton, S.E. Clarke, R. Chenery, and C.R. Wolf (2001). Expression, purification, and biochemical characterization of a human cytochrome P450 CYP2D6-NADPH cytochrome P450 reductase fusion protein. Arch. Biochem. Biophys. 396, 16-24. 

Jackson, C.J., D.C. Lamb, T.H. Marczylo, A.G. Warrilow, N.J. Manning, D.J. Lowe et al. (2002). A novel sterol 14alpha-demethylase/ferredoxin fusion protein (MCCYP51FX) from Methylo-coccus capsulatus represents a new class of the cytochrome P450 superfamily. J. Biol. Chem. 277, 46959 6965. 

Imaoka et found that functional P450 4B1 could be successfully expressed as a fusion protein with NADPH-P450 reductase. They were also successful in developing transgenic mice in which functional P450 was expressed in liver. The authors postulate that expression in the presence of auxiliary proteins (NADPH-P450 reductase, bj) may stabilize P450 4B1 (ref [972]). With these systems, it was possible to demonstrate that... 

Figure 13.2. A proposal for a simplified classification of CYPs with reference to either use of a ferredoxin or alternative electron transport mechanism. Class la— typical bacterial system supported by ferredoxin and ferredoxin reductase, for example, CYPlOl Class Ib—Rhodococcus sp. CYP fusion protein containing a ferredoxin domain Class Ic—Methylococcus capsulatus CYPS 1-ferredoxin fusion . Class 11a— typical eukaryotic CYP/NADPH-cytochrome P450 reductase system Class lib—a fusion protein of a CYP and flavoprotein reductase, for example, P450gM 3 Class IIc—P450 j containing separate flavodoxin and flavodoxin reductase partners . Class fll—standalone functional CYPs, for example, P450jj. ...

Warman AJ, Robinson JW, Luciakova D, Lawrence AD, Marshall KR, Warren MJ, Cheesman MR, Rigby SEJ, Munro AW, McLean KJ (2012) Characterization of Cupriavidus metalUdurans CYP116Bl-a thiocarbamate herbicide oxygenating P450-phthal-ate dioxygenase reductase fusion protein. FEBS J 279 1675-1693... 

Brodhun F, Gobel C, Hornung E, Feussner I (2009) Identification of PpoA from Aspergillus nidulans as a fusion protein of a fatty acid heme dioxygenase/ peroxidase and a cytochrome P450. J Biol Chem 284 11792-11805... 

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